公开(公告)号：EP1032825A1

公开(公告)日：2000-09-06

申请号：EP98955758.2

申请日：1998-11-23

申请人： UNILEVER PLC ,  UNILEVER PLC

发明人： PORTER, Robert, Andrew ,  BADLEY, Robert, Andrew

IPC分类号： G01N27/327 ,  G01N33/543 ,  C07D209/86 ,  C07K16/00

CPC分类号： G01N33/5438 ,  C07D209/86 ,  C07K16/26 ,  C07K16/44 ,  C07K2317/22 ,  C07K2317/31 ,  C07K2317/622 ,  C07K2319/00 ,  Y10S436/806

摘要： Disclosed is a component for a device for detecting the presence of an analyte of interest in a sample, the component comprising an electrically conducting solid support having immobilised thereon a chemical moiety, said chemical moiety comprising an electroactive portion with an electrochemical property capable of being directly modulated in a detectable manner by the binding thereto of a binding partner having a specific binding activity for the electroactive portion, together with apparatus comprising the component, and a method of detecting the presence of an analyte of interest.

公开(公告)号：EP0793806A1

公开(公告)日：1997-09-10

申请号：EP95939289.0

申请日：1995-11-16

申请人： UNILEVER PLC ,  UNILEVER N.V.

发明人： BADLEY, Robert, Andrew ,  BERRY, Mark, John ,  PORTER, Philip ,  WATTAM, Trevor, Anthony, Kenneth

IPC分类号： G01N33

CPC分类号： G01N33/743 ,  G01N33/531 ,  Y10S435/961 ,  Y10T436/10 ,  Y10T436/106664

摘要： An antibody specific for a target analyte is purified by affinity chromatography on a substrate bearing a low-affinity analogue of the target analyte. The antibody is displaced from the substrate by contact with a second analogue of intermediate affinity, which remains complexed with the antibody. This complex can be used in a conventional assay for the target analyte, which displaces the intermediate-affinity analogue. The complexed antibody is rendered more storage-stable because the second analogue protects the antibody binding region.

公开(公告)号：EP1032835A1

公开(公告)日：2000-09-06

申请号：EP98955751.7

申请日：1998-11-23

申请人： UNILEVER PLC ,  UNILEVER N.V.

发明人： BADLEY, Robert, Andrew ,  BERRY, Mark, John ,  HOWELL, Stephen

IPC分类号： G01N33/543

CPC分类号： G01N33/54373

摘要： Disclosed is a method of detecting the presence of an analyte of interest in the sample, the method comprising the steps of: reversibly immobilising on a first surface a displaceable moiety; exposing the first surface to a sample comprising the analyte of interest, the analyte of interest specifically displacing the displaceable moiety from the first surface; causing the displaceable moiety displaced from the first surface to contact a second surface bearing a capture moiety which specifically binds to the displaceable moiety, so as to capture the displaceable moiety on the second surface, said capture generating a detectable signal; and detecting the signal.

公开(公告)号：EP0901630A1

公开(公告)日：1999-03-17

申请号：EP97924008.0

申请日：1997-05-20

申请人： UNILEVER PLC ,  UNILEVER N.V.

发明人： BADLEY, Robert, Andrew ,  BERRY, Mark, John ,  PORTER, Philip ,  WATTAM, Trevor, Anthony, Kenneth

IPC分类号： G01N33

CPC分类号： G01N33/54373 ,  G01N33/558 ,  G01N33/743

摘要： Disclosed is a method of detecting the presence of an analyte of interest in a sample, the method comprising the steps of: contacting the sample with a solid support having reversibly immobilised thereon either a binding partner having specific binding affinity for the analyte, or an analogue of the analyte; and detecting a reduction in the mass of material immobilised on the solid support, said binding partner or analogue being specifically displaced from the solid support in the presence of the analyte of interest, so as to cause a detectable change in the mass of material immobilised thereon. Also disclosed is a device for performing the method.