公开(公告)号：EP2895909A4

公开(公告)日：2016-10-05

申请号：EP13837090

申请日：2013-09-13

申请人： UNIV BRANDEIS ,  INGERSOLL CHARLES G

发明人： INGERSOLL CHARLES G ,  DEROSIER DAVID J ,  TURRIGIANO GINA ,  NAHMANI MARC

IPC分类号： G02B21/28 ,  G02B21/26 ,  G02B21/33

CPC分类号： G02B21/28 ,  G02B21/26 ,  G02B21/32 ,  G02B21/33

摘要： New systems and methods are described for maintaining a desired steady state temperature differential between two objects that may otherwise undergo heat transfer to restore thermal steady state. In one application, a cooling microscope assembly and its use with conventional optical microscopes are described for achieving super-resolution imaging. The assembly allows for the high resolution imaging of samples at cryogenic temperatures while maintaining the temperature of the objective lens above freezing by employing circulation systems and a coupling fluid between the sample and objective Jens.

摘要翻译： 新的系统和方法被描述为保持两个对象之间的期望的稳态温度差也可以以其它方式进行的热传递到热恢复稳定状态。 在一个应用中，冷却显微镜组件及其与传统的光学显微镜使用描述了用于实现超分辨率成像。 该组件允许在低温下采样的高分辨率成像的同时通过采用循环系统和样品和物镜之间的流体耦合维持在冰点以上的物镜的温度。