公开(公告)号：EP3218467A4

公开(公告)日：2018-04-11

申请号：EP15858164

申请日：2015-11-13

申请人： UNIV ILLINOIS

发明人： HA TAEKJIP ,  ARSLAN SINAN

IPC分类号： C12N9/14 ,  C12N9/90

CPC分类号： C12N9/14 ,  C12N9/90 ,  C12P19/34 ,  C12Y306/04012

摘要： Conformationally-constrained helicases having improved activity and strength are provided. Methods of making conformationally-constrained helicases having improved activity and strength are provided. Methods of using conformationally-constrained helicases having improved activity and strength are provided. The present invention is based on the discovery of novel modified helicases that show dramatically enhanced helicase activity and increased strength as compared to unmodified helicases. As described further herein, it has been surprisingly discovered that, by controlling the conformation of certain subdomains such that the helicase remains in a closed form (e.g., by covalently crosslinking the 2B domain to the 1A domain or the 1B domain in a Rep helicase), a highly active and strong form of the helicase is achieved