公开(公告)号：EP0704529A2

公开(公告)日：1996-04-03

申请号：EP95500099.7

申请日：1995-07-06

申请人： UNIVERSIDAD DE OVIEDO

发明人： Francisco Parra c/o Universidad de Oviedo ,  Marin, Maria S. ,  Martin, José M. ,  Casais Rosa Universidad de Oviedo Facultad de Med. ,  Boga, José A.

IPC分类号： C12N15/41 ,  A61K39/125 ,  C07K14/085 ,  C12N15/86

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2770/16022

摘要： Production of the structural antigen (VP60) of the virus of the hemorrhagic disease of the rabbits, RHDV, and its use as a vaccine against said disease. The production of the antigen and the method of vaccination comprise the following stages: a) isolation of the gene that code the structural protein (VP60) of the RHDV; b) cloning of said gene in the baculovirus transfer vector pAcYM1; c) obtention and isolation of the recombinant baculovirus AcVP60; d) infection of Sf9 insect cells with the recombinant baculovirus AcVP60; e) obtention and isolation of the protein VP60 produced by the recombinant baculovirus AcVP60; f) inoculation of the rabbits with the antigen.

摘要翻译： 产生兔子出血性疾病病毒的结构抗原（VP60），RHDV，以及其作为针对所述疾病的疫苗的用途。 抗原的生成和接种方法包括以下阶段：a）分离编码RHDV的结构蛋白（VP60）的基因; b）在杆状病毒转移载体pAcYM1中克隆所述基因; c）获得并分离重组杆状病毒AcVP60; d）用重组杆状病毒AcVP60感染Sf9昆虫细胞; e）获得并分离由重组杆状病毒AcVP60产生的蛋白质VP60; f）用抗原接种兔子。

公开(公告)号：EP0704529A3

公开(公告)日：1998-03-04

申请号：EP95500099.7

申请日：1995-07-06

申请人： UNIVERSIDAD DE OVIEDO

发明人： Francisco Parra c/o Universidad de Oviedo ,  Marin, Maria S. ,  Martin, José M. ,  Casais Rosa Universidad de Oviedo Facultad de Med. ,  Boga, José A.

IPC分类号： C12N15/41 ,  A61K39/125 ,  C07K14/085 ,  C12N15/86

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2770/16022

摘要： Production of the structural antigen (VP60) of the virus of the hemorrhagic disease of the rabbits, RHDV, and its use as a vaccine against said disease. The production of the antigen and the method of vaccination comprise the following stages: a) isolation of the gene that code the structural protein (VP60) of the RHDV; b) cloning of said gene in the baculovirus transfer vector pAcYM1; c) obtention and isolation of the recombinant baculovirus AcVP60; d) infection of Sf9 insect cells with the recombinant baculovirus AcVP60; e) obtention and isolation of the protein VP60 produced by the recombinant baculovirus AcVP60; f) inoculation of the rabbits with the antigen.