公开(公告)号：EP1228196A1

公开(公告)日：2002-08-07

申请号：EP00971584.8

申请日：2000-10-26

申请人： UNIVERSITY COLLEGE LONDON

发明人： ANDERSON, Robert, James ,  PRENTICE, Hugh, Grant

IPC分类号： C12N7/02

CPC分类号： C12N7/00 ,  C12N2750/14151

摘要： A method for purifying an adeno-associated virus, which method comprises: 1) applying a sample of impure virus to an affinity matrix under conditions to bind the adeno-associated virus to the matrix, wherein the affinity matrix comprises an affinant capable of competing with heparan sulphate for binding to the virus; 2) eluting contaminants from the matrix to produce a virus-rich matrix; 3) eluting virus from the virus-rich matrix; 4) treating the eluted virus with a protease incapable of digesting intact virus to digest any proteinaceous contaminants; and 5) separating intact virus from the digested proteinaceous contaminants.