公开(公告)号：EP2917357A4

公开(公告)日：2016-06-15

申请号：EP13853775

申请日：2013-11-04

申请人： US AGRICULTURE ,  DUPONT IND BIOSCIENCES

发明人： JOHNSTON DAVID ,  HICKS KEVIN B ,  MOREAU ROBERT A ,  SHETTY JAYARAMA K

IPC分类号： C12P7/64 ,  C11B1/02 ,  C12N9/26

CPC分类号： C12P7/64 ,  C11B1/025 ,  C11B1/10 ,  C12P7/06 ,  C12Y302/01004 ,  C12Y304/00 ,  Y02E50/17

摘要： Disclosed are methods for obtaining oil from maize, involving grinding maize kernels to form flour, adding water to the flour to form a slurry, and incubating the slurry with α-amylase for about 10 minutes to about 180 minutes at a temperature of about 75° to about 120° C. and at a pH of about 3 to about 7 to form a mash, cooling the mash to about 15° C. to about 40° C. and adding a nitrogen source, glucoamylase, yeast, acid protease, and cell-wall polysaccharide-degrading enzymes to form a beer containing ethanol and oil, wherein the beer has a pH of about 3 to about 7, and recovering oil from the beer.