公开(公告)号：EP2911683A1

公开(公告)日：2015-09-02

申请号：EP13848625.3

申请日：2013-10-23

申请人： Ubivac Inc. ,  Providence Health & Services - Oregon

发明人： HILTON, Traci ,  AUNG, Sandra ,  VAN DE VEN, Rieneke ,  PAUSTIAN, Christopher ,  MOUDGIL, Tarsem ,  DUBAY, Christopher ,  TWITTY, Christopher ,  HU, Hong-Ming ,  FOX, Bernard A.

IPC分类号： A61K38/20 ,  A61K38/21 ,  A61K38/19 ,  A61P35/00

CPC分类号： C12Q1/6886 ,  A61K39/0011 ,  A61K39/39 ,  A61K2039/55522 ,  A61K2039/55561 ,  C12Q2600/136 ,  C12Q2600/158 ,  G01N33/5023 ,  G01N33/5047 ,  G01N33/505 ,  G01N2500/10

摘要： A method is presented for screening cells that produce allogeneic autophagosome enriched compositions able to induce expression of a selective marker on a subpopulation of peripheral blood mononuclear cells, the method comprising contacting a cell with a proteasome inhibitor, contacting the cell with a lysosome inhibitor, harvesting the resulting autophagosomes, determining a molecular signature of the harvested autophagosomes, and selecting cells that divert one or more Toll-like receptor agonist and/or one or more molecular chaperones to the harvested autophagosomes. By screening for cells that divert one or more Toll-like receptor agonist and/or one or more molecular chaperones to the harvested autophagosomes, enriched populations of autophagosomes may be generated which may illicit a specific immune response against numerous cancer types. In this way, an allogeneic, off-the-shelf cancer vaccine may be produced and made available to be administered in order to stimulate a targeted immune response in patients bearing different tumor types.