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公开(公告)号：EP3020805B1
公开(公告)日：2018-09-12
申请号：EP14822858.8
申请日：2014-05-21
申请人： Umn Pharma Inc. , Vesikari, Timo , Blazevic, Vesna
发明人： OKADA, Masahiro , MUKAI, Akiko , NISHINO, Tomonori , ARINOBU, Daisuke , ITO, Hiroyuki , SATOH, Mamoru
CPC分类号： C12N7/00 , A61K39/12 , A61K2039/5256 , A61K2039/5258 , C07K14/005 , C12N2710/14043 , C12N2770/16022 , C12N2770/16023 , C12N2770/16034 , C12N2770/16051
摘要： The present invention aims at establishing a means for obtaining highly pure virus-like particles by a simple method. There is provided a production method for culture containing virus-like particles, comprising: transforming insect cells with baculovirus vector containing a viral nucleic acid sequence; and cultivating the insect cells, wherein the culture is obtained after continuing cultivating the insect cells until the viability of the insect cells reaches 10% or less.
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公开(公告)号：EP3020721B1
公开(公告)日：2019-07-03
申请号：EP14822108.8
申请日：2014-05-21
申请人： Umn Pharma Inc. , Vesikari, Timo , Blazevic, Vesna
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公开(公告)号：EP3020805A1
公开(公告)日：2016-05-18
申请号：EP14822858.8
申请日：2014-05-21
申请人： Umn Pharma Inc. , Vesikari, Timo , Blazevic, Vesna
发明人： OKADA, Masahiro , MUKAI, Akiko , NISHINO, Tomonori , ARINOBU, Daisuke , ITO, Hiroyuki , SATOH, Mamoru
CPC分类号： C12N7/00 , A61K39/12 , A61K2039/5256 , A61K2039/5258 , C07K14/005 , C12N2710/14043 , C12N2770/16022 , C12N2770/16023 , C12N2770/16034 , C12N2770/16051
摘要： The present invention aims at establishing a means for obtaining highly pure virus-like particles by a simple method. There is provided a production method for culture containing virus-like particles, comprising: transforming insect cells with baculovirus vector containing a viral nucleic acid sequence; and cultivating the insect cells, wherein the culture is obtained after continuing cultivating the insect cells until the viability of the insect cells reaches 10% or less.
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4.发明公开
公开(公告)号：EP3020721A1
公开(公告)日：2016-05-18
申请号：EP14822108.8
申请日：2014-05-21
申请人： Umn Pharma Inc. , Vesikari, Timo , Blazevic, Vesna
发明人： OKADA, Masahiro , MUKAI, Akiko , NISHINO, Tomonori , ARINOBU, Daisuke , ITO, Hiroyuki , SATOH, Mamoru
CPC分类号： C12N7/00 , C12N2770/16023 , C12N2770/16051
摘要： The present invention aims at establishing a novel means for purifying norovirus VLPs. There is provided a purification method of norovirus virus-like particles, comprising: contacting solution containing norovirus virus-like particles with support for hydroxyapatite chromatography, binding the virus-like particles to the support, subsequently washing the support with buffer, and then eluting the virus-like particles from the support with buffer containing phosphate, wherein the phosphate concentration of the buffer used for elution is less than 10 mM.
摘要翻译： 本发明旨在建立一种纯化诺如病毒VLPs的新方法。 提供了一种诺如病毒病毒样颗粒的纯化方法，其包括：将含有诺如病毒病毒样颗粒的溶液与支持物用于羟基磷灰石层析，将病毒样颗粒结合到载体上，随后用缓冲液洗涤载体， 来自含有磷酸盐缓冲液的载体的病毒样颗粒，其中用于洗脱的缓冲液的磷酸盐浓度小于10mM。
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