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    HLA-B GENOTYPING METHOD AND KIT BASED ON REAL-TIME PCR
    1.
    发明公开
    HLA-B GENOTYPING METHOD AND KIT BASED ON REAL-TIME PCR 有权
    HLA-B基因组突变体免疫球蛋白基因

    公开(公告)号：EP1964929A1

    公开(公告)日：2008-09-03

    申请号：EP06841799.7

    申请日：2006-12-12

    申请人： Universitat Autonoma de Barcelona ,  Banc De Sang I Teixits

    发明人： JUAN OTERO, Manuel ,  CASAMITJANA PONCES, Natàlia ,  PALOU RIVERA, Eduardo ,  PUJOL BORRELL, Ricardo ,  FANER CANET, María, Rosa

    IPC分类号： C12Q1/68 ,  G01N33/58

    CPC分类号： C12Q1/6881 ,  C12Q2600/156

    摘要： Method and kit based on real-time PCR with specific primers and probes that allow to achieve a high degree of subtyping of the complete HLA-B locus. The main advantages are the greater speed (65 minutes, including the interpretation); the ease of automation, since only eighteen tubes are necessary to obtain a good level of resolution (typing of 300 groups); reduction of the total cost per test thanks to the ease of automation and the simplicity; a surprisingly high degree of allele definition is achieved; and the risk of sample contamination is reduced because the amplified products always remain in the tubes and no post-PCR steps are necessary.

    摘要翻译： 基于实时PCR的特异性引物和探针的方法和试剂盒，其允许实现完整的HLA-B基因座的高度亚型化。 主要优点是速度更快（65分钟，包括解释）; 自动化的便利性，因为只有十八根管是获得良好的分辨率（打300个组）所必需的; 由于易于自动化和简单性，每次测试的总成本降低; 达到惊人的高度等位基因定义; 并且样品污染的风险降低，因为扩增产物总是保留在管中，并且不需要后PCR步骤。