公开(公告)号：EP1447412A1

公开(公告)日：2004-08-18

申请号：EP03075460.0

申请日：2003-02-17

申请人： Universiteit Utrecht Holding B.V.

发明人： Braakman, Lina Jantina ,  van Anken, Eelco

IPC分类号： C07K14/47 ,  G01N27/447

CPC分类号： C07K14/47 ,  G01N33/5076 ,  G01N33/6803

摘要： The invention provides a novel method of analysing the proteome of a cell. The method allows the correlation of expression of proteins with the activity of compartments in the cell and the association of thus far unidentified proteins with(in) such compartments. This gives the artisan a powerful tool in the elucidation of the function of proteins thus identified. According to this method a new family of proteins was identified and the function thereof determined and confirmed by experiments. The family termed smERp belongs to the oxidoreductase proteins, but is distinct therefrom in their activity and internal structure. The invention further identifies several other proteins and their function, together with methods wherein the identified proteins can be manipulated to obtain, or gain further functionality. The invention also provides methods wherein proteins identified in the present invention are used for the production of proteins of interest.

摘要翻译： 本发明提供了一种分析细胞蛋白质组的新方法。 该方法允许蛋白质的表达与细胞中室的活性和迄今未鉴别的蛋白质与（在）这种隔室中的相互作用的相关性。 这给了工匠一个强大的工具，用于阐明所鉴定的蛋白质的功能。 根据该方法，鉴定出新的蛋白质家族，并通过实验确定并证实其功能。 称为smERP的家族属于氧化还原酶蛋白，但与其活性和内部结构不同。 本发明进一步鉴定了其他蛋白质及其功能，以及可以操作鉴定的蛋白质以获得或获得更多功能的方法。 本发明还提供了将本发明中鉴定的蛋白质用于生产感兴趣的蛋白质的方法。