公开(公告)号：EP2291651A1

公开(公告)日：2011-03-09

申请号：EP08763476.2

申请日：2008-06-25

申请人： University College Cork-National University of Ireland, Cork

发明人： PAPKOVSKY, Dmitri ,  JASIONEK, Grzegorz ,  ZHDANOV, Alexander

IPC分类号： G01N33/50

CPC分类号： G01N33/5014

摘要： A method of assessing toxicity of a candidate agent to a sample of cells comprises the steps of providing a sample of cells, exposing the cells to the candidate agent for a suitable period of time, assaying the cells to measure data for at least one parameter of cellular function; and correlating the measured data of the at least one parameter of cellular function with toxicity, wherein the step of exposing the cells to the candidate agent is carried out in the presence of a reagent capable of facilitating transport of the candidate agent into the cell. The transport reagent may be an endocytosis, pinocytosis inducing agent, a peptide, or a liposome. The at least one parameter of cellular function may be selected from the group consisting of: cell viability; proliferation rate; membrane integrity; and a metabolic parameter. Also described is a method of generating a toxicity signature for a candidate agent comprising the step of carrying out the method of the invention for a plurality of cellular function parameters, and compiling the measured data for each of the cellular function parameters to provide a toxicity signature.

公开(公告)号：EP2630488B1

公开(公告)日：2016-08-17

申请号：EP10779731.8

申请日：2010-10-22

申请人： University College Cork, National University of Ireland, Cork

发明人： PAPKOVSKY, Dmitri ,  FERCHER, Andreas ,  KLIMANT, Ingo ,  BORISOV, Sergey ,  ZHDANOV, Alexander

IPC分类号： G01N33/50 ,  G01N33/84 ,  G01N21/64 ,  G01N33/58

CPC分类号： G01N21/6486 ,  B82Y30/00 ,  G01N33/5005 ,  G01N33/587 ,  G01N33/84

公开(公告)号：EP2630488A1

公开(公告)日：2013-08-28

申请号：EP10779731.8

申请日：2010-10-22

申请人： University College Cork, National University of Ireland, Cork

发明人： PAPKOVSKY, Dmitri ,  FERCHER, Andreas ,  KLIMANT, Ingo ,  BORISOV, Sergey ,  ZHDANOV, Alexander

IPC分类号： G01N33/50 ,  G01N33/84 ,  G01N33/58 ,  G01N31/22

CPC分类号： G01N21/6486 ,  B82Y30/00 ,  G01N33/5005 ,  G01N33/587 ,  G01N33/84

摘要： The invention is based on the use of photoluminescent probes for intracellular sensing of oxygen, especially assaying intracellular oxygen concentration. The photoluminescent probe comprises a suspension of polymeric particles having an average diameter in the 20nm to 100nm range, formed from an amphiphilic cationic co-polymer which is oriented in the formed particle to provide a hydrophobic core and a hydrophilic shell. The probe includes a hydrophobic oxygen-sensitive photoluminescent dye such as Pt-tetrakis (pentafluorophenyl) porphine, PtPFPP embedded in the hydrophobic core of the particle, and the co-polymer includes quaternary ammonium groups which provide hydrophilic and cationic character to the particle shell. The photoluminescent probe, which in use is provided in the form of an aqueous suspension of probe, is incubated with live mammalian cells in a suitable growth medium for a period of time to allow the probe particles passively load into the cells. Oxygen can then be sensed by detecting a photoluminescent signal of the photoluminescent probe, which can be correlated with oxygen status, for example oxygen concentration or changes in oxygen concentration/levels, using existing techniques.