公开(公告)号：EP4231010A1

公开(公告)日：2023-08-23

申请号：EP22157103.7

申请日：2022-02-16

申请人： University College Cork-National University of Ireland Cork

发明人： Papkovsky, Dmitri ,  Zhdanov, Alexander ,  Li, Liang

IPC分类号： G01N31/22 ,  G01N21/64 ,  G01N21/77

摘要： An optochemical sensor comprises a substrate coated with a solid-state optically active pH-sensitive material comprising a hydrophobic plasticised polymer matrix, a protonable photoluminescent dye selected from the group consisting of a hydrophobic metal-free porphyrin and a porphyrin-ketone, and a hydrophobic proton transfer reagent, in which the hydrophobic proton-transfer reagent comprises a salt of a fluorinated derivative of tetraphenylborate. The salt of the substituted tetraphenylborate derivative is selected from a salt of tetrakis[3,5-bis(trifluoromethyl)phenyl]borate and a salt of tetrakis-(pentafluorophenyl)borate. The hydrophobic polymer comprises poly(n-butylacrylate) or poly(vinylchloride) and a suitable plasticiser. The use of substituted fluorinated TCPB analogues, in particular sodium salt of tetrakis[3,5-bis(trifluoromethyl)phenyl]borate, as a proton transfer agent (PTA), is seen to have excellent solubility in plasticised PVC and poly(n-butylacrylate) matrices and low cytotoxic action on cells. Moreover, by changing the concentration of this PTA in the sensor, one can tune the sensor pKa values and measured range of pH values, to cover the most important physiological range of pH (7.5 - 6.0) and ECA rates.