公开(公告)号：EP2360252B8

公开(公告)日：2017-04-12

申请号：EP10186177.1

申请日：1997-10-30

申请人： University Of Iowa Research Foundation ,  THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by the SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES

发明人： Krieg, Arthur ,  Klinman, Dennis ,  Weiner, George

IPC分类号： C12N15/11 ,  C07H21/00 ,  C07H21/04 ,  A61K31/7125 ,  A61P31/00 ,  A61P35/00

CPC分类号： A61K39/00 ,  A61K31/00 ,  A61K31/4706 ,  A61K31/7048 ,  A61K31/711 ,  A61K31/7125 ,  A61K39/39 ,  A61K2039/55561 ,  C07H21/00 ,  C12N15/117 ,  C12N2310/17 ,  C12N2310/315 ,  C12Q1/68 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/466 ,  Y02A50/54

摘要： Nucleic acid sequences containing unmethylated CpG dinucleotides that modulate an immune response including stimulating a Th1 pattern of immune activation, cytokine production, NK lytic activity, and B cell proliferation are disclosed. The sequences are also useful as synthetic adjuvant.

摘要翻译： 公开了含有调节免疫应答的非甲基化CpG二核苷酸的核酸序列，包括刺激免疫激活，细胞因子产生，NK溶解活性和B细胞增殖的Th1模式。 序列也可用作合成佐剂。

公开(公告)号：EP2360252A1

公开(公告)日：2011-08-24

申请号：EP10186177.1

申请日：1997-10-30

申请人： UNIVERSITY OF IOWA RESEARCH FOUNDATION ,  THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by the SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES

发明人： Krieg, Arthur ,  Klinman, Dennis ,  Weiner, George

IPC分类号： C12N15/11 ,  C07H21/00 ,  C07H21/04 ,  A61K31/7125 ,  A61P31/00 ,  A61P35/00

CPC分类号： A61K39/00 ,  A61K31/00 ,  A61K31/4706 ,  A61K31/7048 ,  A61K31/711 ,  A61K31/7125 ,  A61K39/39 ,  A61K2039/55561 ,  C07H21/00 ,  C12N15/117 ,  C12N2310/17 ,  C12N2310/315 ,  C12Q1/68 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/466 ,  Y02A50/54

摘要： An isolated immunostimulatory oligonucleotide comprising two or more unmethylated CpG dinucleotides, and having a sequence including the following formula:

        5'N 1 X 1 X 2 CGTTN 2 3'

wherein the immunostimulatory oligonucleotide includes a modified backbone, at least one nucleotide separates consecutive CpGs, X 1 X 2 is GpT or ApT, N 1 is any nucleotide that includes a 5' TC or TG, N 1 + N 2 is from 0-26 bases, and the immunostimulatory oligonucleotide is from 8-30 bases in length and begins with TC or TG at the 5' end, with the proviso that neither N 1 nor N 2 contains a CCGG quadmer or more than one CCG or CGG trimer.

摘要翻译： 包含两个或更多个未甲基化的CpG二核苷酸的分离的免疫刺激性寡核苷酸，并且具有包括下式的序列：ƒ€ƒ€ƒ€ƒ€ƒ€ƒ5'N1 X 1 X 2 CGTTN 2 3'其中 免疫刺激性寡核苷酸包括修饰的主链，至少一个核苷酸分离连续的CpG，X 1 X 2是GpT或ApT，N 1是包括5'TC或TG的任何核苷酸，N 1 + N 2是0-26个碱基， 免疫刺激寡核苷酸长度为8-30个碱基，以5'末端的TC或TG开始，条件是N 1和N 2均不含有CCGG四聚体或多于一个CCG或CGG三聚体。

公开(公告)号：EP2360252B1

公开(公告)日：2017-02-08

申请号：EP10186177.1

申请日：1997-10-30

申请人： UNIVERSITY OF IOWA RESEARCH FOUNDATION ,  THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by the SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES

发明人： Krieg, Arthur ,  Klinman, Dennis ,  Weiner, George

IPC分类号： C12N15/11 ,  C07H21/00 ,  C07H21/04 ,  A61K31/7125 ,  A61P31/00 ,  A61P35/00

CPC分类号： A61K39/00 ,  A61K31/00 ,  A61K31/4706 ,  A61K31/7048 ,  A61K31/711 ,  A61K31/7125 ,  A61K39/39 ,  A61K2039/55561 ,  C07H21/00 ,  C12N15/117 ,  C12N2310/17 ,  C12N2310/315 ,  C12Q1/68 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/466 ,  Y02A50/54

摘要： Nucleic acid sequences containing unmethylated CpG dinucleotides that modulate an immune response including stimulating a Th1 pattern of immune activation, cytokine production, NK lytic activity, and B cell proliferation are disclosed. The sequences are also useful as synthetic adjuvant.

公开(公告)号：EP2172216A2

公开(公告)日：2010-04-07

申请号：EP09166906.9

申请日：1998-03-10

申请人： Ottawa Hospital Research Institute ,  Coley Pharmaceutical GmbH ,  University of Iowa Research Foundation ,  The United States of America as represented by the Secretary Department of Health and Human Services

发明人： Davis, Heather ,  Krieg, Arthur ,  Schorr, Joachim ,  Klinman, Dennis

IPC分类号： A61K39/39

CPC分类号： A61K39/292 ,  A61K39/12 ,  A61K39/39 ,  A61K2039/523 ,  A61K2039/53 ,  A61K2039/55505 ,  A61K2039/5555 ,  A61K2039/55561 ,  A61K2039/57 ,  C12N2730/10134

摘要： The present invention is based on the finding that nucleic acids containing at least one unmethylated cytosine-guanine (CpG) dinucleotide affect immune responses in a subject. These nucleic acids containing at least one unmethylated cytosine-guanine (CpG) dinucleotide can be used to induce an immune response in a subject. The method includes administering to the subject a therapeutically effective amount of nucleic acid encoding an antigenic polypeptide, and a therapeutically effective amount of an oligonucleotide containing at least one unmethylated CpG dinucleotide. The invention also provides a method for treating a subject having or at risk of having viral-mediated disorder, comprising administering to the subject a therapeutically effective amount of a nucleic acid encoding an antigenic polypeptide and an effective amount of an oligonucleotide containing at least one unmethylated CpG dinucleotide

摘要翻译： 本发明基于以下发现：含有至少一种未甲基化的胞嘧啶 - 鸟嘌呤（CpG）二核苷酸的核酸影响受试者的免疫应答。 这些含有至少一个未甲基化的胞嘧啶 - 鸟嘌呤（CpG）二核苷酸的核酸可用于在受试者中诱导免疫应答。 该方法包括向受试者施用治疗有效量的编码抗原性多肽的核酸和治疗有效量的含有至少一种未甲基化的CpG二核苷酸的寡核苷酸。 本发明还提供了用于治疗患有或有风险患有病毒介导的疾病的对象的方法，包括向对象施用治疗有效量的编码抗原性多肽的核酸和有效量的含有至少一种未甲基化的寡核苷酸 CpG二核苷酸

公开(公告)号：EP2172216A3

公开(公告)日：2010-11-24

申请号：EP09166906.9

申请日：1998-03-10

申请人： Ottawa Hospital Research Institute ,  Coley Pharmaceutical GmbH ,  University of Iowa Research Foundation ,  The United States of America as represented by the Secretary Department of Health and Human Services

发明人： Davis, Heather ,  Krieg, Arthur ,  Schorr, Joachim ,  Klinman, Dennis

IPC分类号： A61K39/39

CPC分类号： A61K39/292 ,  A61K39/12 ,  A61K39/39 ,  A61K2039/523 ,  A61K2039/53 ,  A61K2039/55505 ,  A61K2039/5555 ,  A61K2039/55561 ,  A61K2039/57 ,  C12N2730/10134

摘要： The present invention is based on the finding that nucleic acids containing at least one unmethylated cytosine-guanine (CpG) dinucleotide affect immune responses in a subject. These nucleic acids containing at least one unmethylated cytosine-guanine (CpG) dinucleotide can be used to induce an immune response in a subject. The method includes administering to the subject a therapeutically effective amount of nucleic acid encoding an antigenic polypeptide, and a therapeutically effective amount of an oligonucleotide containing at least one unmethylated CpG dinucleotide. The invention also provides a method for treating a subject having or at risk of having viral-mediated disorder, comprising administering to the subject a therapeutically effective amount of a nucleic acid encoding an antigenic polypeptide and an effective amount of an oligonucleotide containing at least one unmethylated CpG dinucleotide

公开(公告)号：EP2322615A1

公开(公告)日：2011-05-18

申请号：EP10186108.6

申请日：1997-10-30

申请人： UNIVERSITY OF IOWA RESEARCH FOUNDATION ,  THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, the Department of Health and Human Services,

发明人： Weiner, George ,  Krieg, Arthur ,  Klinman, Dennis

IPC分类号： C12N15/11 ,  C07H21/00 ,  C07H21/04 ,  A61K31/7125 ,  A61P31/00 ,  A61P35/00

CPC分类号： A61K39/00 ,  A61K31/00 ,  A61K31/4706 ,  A61K31/7048 ,  A61K31/711 ,  A61K31/7125 ,  A61K39/39 ,  A61K2039/55561 ,  C07H21/00 ,  C12N15/117 ,  C12N2310/17 ,  C12N2310/315 ,  C12Q1/68 ,  Y02A50/412 ,  Y02A50/464 ,  Y02A50/466 ,  Y02A50/54

摘要： An immuostimulatory nucleic acid containing an unmethylated CpG dinucleotide for use in a method of treating or preventing an allergy in a subject, wherein an effective dose of the immunostimulatory nucleic acid alone is to be administered to the subject.

摘要翻译： 一种用于治疗或预防受试者过敏的方法的非甲基化CpG二核苷酸的免疫刺激性核酸，其中单独施用免疫刺激性核酸的有效剂量。

公开(公告)号：EP1688147A1

公开(公告)日：2006-08-09

申请号：EP05111935.2

申请日：2000-09-27

申请人： Coley Pharmaceutical Group, Inc. ,  UNIVERSITY OF IOWA RESEARCH FOUNDATION

发明人： Hartmann, Gunther, Dept. of Internal Medicine ,  Bratzler, Robert L ,  Krieg, Arthur

IPC分类号： A61K38/21 ,  A61K35/28 ,  C12N5/08 ,  A61P35/00 ,  A61K31/7088 ,  A61K38/19

CPC分类号： C12N5/0639 ,  A61K38/212 ,  A61K2035/124 ,  C12N2501/056 ,  C12N2501/10 ,  C12N2501/22 ,  C12N2501/23 ,  A61K2300/00

摘要： Methods and compositions are provided for extending the clinical utility of IFN-α in the treatment of a variety of viral and proliferative disorders. Among other aspects, the invention provides methods which increase the efficacy of IFN-α treatment and reduce IFN-α treatment-related side effects. In addition, methods are provides for supporting the survival and for activating natural interferon producing cells (IPCs) in vitro without exogenous IL-3 or GM-CSF. The invention is based on the discovery that certain CpG and non-CpG ISNAs promote survival and stimulation of IPCs.

摘要翻译： 提供了方法和组合物，用于延长IFN-±在治疗各种病毒和增殖性疾病中的临床应用。 在其它方面，本发明提供了增加IFN-α治疗功效并减少IFN-治疗相关副作用的方法。 此外，还提供了用于支持生存和在体外激活天然干扰素产生细胞（IPC）的方法，而不需要外源IL-3或GM-CSF。 本发明基于某些CpG和非CpG ISNA促进IPC的存活和刺激的发现。