公开(公告)号：EP0981731A1

公开(公告)日：2000-03-01

申请号：EP98909080.8

申请日：1998-03-09

申请人： University of Washington

发明人： WEIGL, Bernhard ,  YAGER, Paul ,  BRODY, James, P.

IPC分类号： G01N15/14

CPC分类号： G01N15/1404 ,  Y10S366/03 ,  Y10T436/117497 ,  Y10T436/118339

摘要： The present invention provides a sheath flow module made from a first plate of material having formed therein a laminar fluid flow channel; at least two inlets, each inlet joining the laminar flow channel at a junction, the first inlet junction being wider than the second inlet junction, and an outlet from the flow channel. A second plate, e.g. a transparent cover plate, seals the module and allows for optical measurements. A first inlet allows for introduction of a first fluid into the flow channel. The first fluid is the sheath fluid. A second inlet allows for introduction of a second fluid into the sheath fluid while it is flowing through the flow channel. The second fluid is the center fluid. Because the second inlet junction is narrower than the first inlet junction, the center fluid becomes surrounded on both sides by the sheath fluid. After all fluids have been introduced and sheath flow has been achieved, the depth of the flow channel can be decreased, leading to vertical hydrodynamic focusing. Optionally, the width of the flow channel can be decreased, leading to horizontal hydrodynamic focusing. The decrease in depth and width can be gradual or abrupt. The device of the present invention can function in two modes, the sheath flow mode and the particle injector mode, depending on the relative densities of the sheath fluid, the center fluid, and any particles in either fluid.

摘要翻译： 本发明提供了一种由第一板材形成的鞘流模块，其中形成有层流体流动通道; 至少两个入口，每个入口在接合处连接层流通道，第一入口接头宽于第二入口接头，以及流道的出口。 第二块板例如 一个透明的盖板，密封模块并允许进行光学测量。 第一入口允许将第一流体引入流动通道。 第一种流体是鞘液。 当第二流体流过流动通道时，第二入口允许将第二流体引入鞘液中。 第二种流体是中心流体。 由于第二入口接头比第一入口接头窄，中心流体在两侧被鞘液包围。 在所有流体被引入并且已经实现鞘流后，流动通道的深度可以减小，导致垂直流体动力学聚焦。 可选地，流动通道的宽度可以减小，导致水平流体动力学聚焦。 深度和宽度的减小可以是渐变或突变的。 根据鞘液，中心流体以及任一流体中的任何颗粒的相对密度，本发明的装置可以以两种模式起作用，即鞘流模式和颗粒注射器模式。