公开(公告)号：EP1446488A2

公开(公告)日：2004-08-18

申请号：EP02802938.7

申请日：2002-10-30

申请人： VIRxSYS Corporation

发明人： CHANG, Yung-Nien ,  NI, Yajin ,  DROPULIC, Boro

IPC分类号： C12N15/63

CPC分类号： C12N7/00 ,  C12N15/635 ,  C12N2740/16052 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2740/16322

摘要： The present invention provides nucleic acid constructs, expression systems, and methods relating to the regulation of gene expression. The invention may be applied to regulate the expression of any coding sequence of interest, including those coding for viral components necessary for the packaging of viral particles.

公开(公告)号：EP1315823A2

公开(公告)日：2003-06-04

申请号：EP01966434.1

申请日：2001-08-29

申请人： VIRxSYS Corporation

发明人： HUMEAU, Laurent ,  HAN, Wei ,  LU, Xiaobin ,  SLEPUSHKIN, Vladimir ,  LESHER, Mechelle ,  DAVIS, Brian ,  CHANG, Yung-Nien ,  DROPULIC, Boro

IPC分类号： C12N15/87 ,  C12N15/86 ,  A61K48/00

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2740/16043

摘要： The present invention provides methods, as well as compositions related thereto, for the efficient transduction of cells using viral vectors. The efficiency of transduction is increased by contacting the cell to be transduced with one or more molecules that bind the cell surface. Contact with a cell surface binding molecule may occur before, after, or simultaneously with contact between the viral vector and the cell. The transduced vectors may be constructed to express a gene of interest, permitting the transduced cells to be used as therapeutic and prophylactic agents.