公开(公告)号：EP1309904A1

公开(公告)日：2003-05-14

申请号：EP01913279.4

申请日：2001-03-02

申请人： Valentis, Inc.

发明人： NICOL, François ,  WANG, Jijun ,  COLEMAN, Michael ,  MACLAUGHLIN, Fiona ,  ROLLAND, Alain

IPC分类号： G06D1/00

CPC分类号： C12N15/87 ,  A61K38/00 ,  A61K38/208 ,  A61K39/00 ,  A61K47/34 ,  A61K48/00 ,  A61K48/0008 ,  A61K48/0075 ,  A61K2039/53 ,  C07K14/475

公开(公告)号：EP0981378A1

公开(公告)日：2000-03-01

申请号：EP98906110.6

申请日：1998-02-04

申请人： Valentis Inc.

发明人： COLEMAN, Michael

IPC分类号： A61K48/00

CPC分类号： A61K38/30 ,  A61K38/25 ,  A61K38/27 ,  A61K48/00

摘要： The invention is directed in part towards methods of treating urinary incontinence using gene therapy techniques. The methods provide for the delivery and expression of growth factors or neurotrophic factors in mammalian tissues.

公开(公告)号：EP0943003A1

公开(公告)日：1999-09-22

申请号：EP97950737.0

申请日：1997-12-01

申请人： Valentis Inc. ,  THE BAYLOR COLLEGE OF MEDICINE

发明人： COLEMAN, Michael ,  SCHWARTZ, Robert ,  DEMAYO, Francesco, J.

IPC分类号： A01K67 ,  A61K31 ,  A61K38 ,  A61K48 ,  A61P3 ,  A61P5 ,  A61P19 ,  A61P21 ,  C07K14 ,  C12N5 ,  C12N15

CPC分类号： C12N15/85 ,  A01K2217/05 ,  A61K38/00 ,  C07K14/65 ,  C12N2830/001 ,  C12N2830/42 ,  C12N2830/90

摘要： This invention relates to gene delivery and expression, including gene therapy, by using vectors which encode stable mRNA and methods of using such vectors. In particular, this invention relates to vectors which establish controlled expression of recombinant IGF-I genes within tissues at certain levels. The vector includes a 5' flanking region which includes necessary sequences for expression of a nucleic acid cassette, a 3' flanking region including a 3' UTR and/or 3' NCR, and a linker which connects the 5' flanking region to a nucleic acid sequence. The linker has a position for inserting a nucleic acid cassette. The linker does not contain the coding sequence of a gene that the linker is naturally associated with. The 3' flanking region is 3' to the position for inserting the nucleic acid cassette. The expression vectors of the present invention can also be regulated by a regulatory system and/or constructed with a coating.

公开(公告)号：EP0943003B1

公开(公告)日：2004-11-17

申请号：EP97950737.3

申请日：1997-12-01

申请人： Valentis Inc. ,  THE BAYLOR COLLEGE OF MEDICINE

发明人： COLEMAN, Michael ,  SCHWARTZ, Robert ,  DEMAYO, Francesco, J.

IPC分类号： C12N15/85 ,  C07K14/65 ,  A61K48/00 ,  A01K67/027

CPC分类号： C12N15/85 ,  A01K2217/05 ,  A61K38/00 ,  C07K14/65 ,  C12N2830/001 ,  C12N2830/42 ,  C12N2830/90

摘要： This invention relates to gene delivery and expression, including gene therapy, by using vectors which encode stable mRNA and methods of using such vectors. In particular, this invention relates to vectors which establish controlled expression of recombinant IGF-I genes within tissues at certain levels. The vector includes a 5' flanking region which includes necessary sequences for expression of a nucleic acid cassette, a 3' flanking region including a 3' UTR and/or 3' NCR, and a linker which connects the 5' flanking region to a nucleic acid sequence. The linker has a position for inserting a nucleic acid cassette. The linker does not contain the coding sequence of a gene that the linker is naturally associated with. The 3' flanking region is 3' to the position for inserting the nucleic acid cassette. The expression vectors of the present invention can also be regulated by a regulatory system and/or constructed with a coating.