公开(公告)号：EP0876428B1

公开(公告)日：2004-11-17

申请号：EP96934591.7

申请日：1996-10-08

申请人： Visen Medical, Inc.

发明人： DELLA CIANA, Leopoldo ,  GRIGNANI, Andrea ,  CASSULLO, Mariacristina ,  CAPUTO, Giuseppe

IPC分类号： C09B23/00 ,  G01N33/533 ,  C12Q1/68

CPC分类号： G01N33/533 ,  C09B23/0066 ,  C09B23/02 ,  Y10S436/80 ,  Y10S436/817

摘要： The present invention is directed to fluorescent dyes and its valence tautomers of formula (I), wherein Q represents a conjugated moiety that increases the fluorescent quantum yield of the compound; R1 is a functionalized group of the formula -(CH2)jY, wherein Y is selected from the group consisting of SO3H, COOH, NH2, CHO, NCS, epoxy, phthalimido, and COOZ, wherein Z represents a leaving group; R2 is a functionalized group of the formula -(CH2)kY', wherein Y' is selected from the group consisting of SO3H, COOH, NH2, CHO, NCS, epoxy, phthalimido, and COOZ, wherein Z represents a leaving group; M is a counterion selected from the group consisting of ammonium, alkali metal cations, and alkaline earth metal cations; n = 1 to 4; m = 1 to 4; j = 2 to 10; and k = 2 to 10.