公开(公告)号：EP3336192A1

公开(公告)日：2018-06-20

申请号：EP18153133.6

申请日：2014-11-12

申请人： Vrije Universiteit Brussel

发明人： HEIRMAN, Carlo ,  THIELEMANS, Kristiaan

IPC分类号： C12N15/85 ,  A61K39/00 ,  A61K31/7088

CPC分类号： A61K48/0066 ,  A61K31/7105 ,  A61K39/0011 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/55516 ,  C07K14/70575 ,  C07K14/70596 ,  C12N15/85 ,  C12N2320/52 ,  C12N2830/50 ,  C12N2840/105

摘要： The present invention in general relates to an improved RNA transcription vector, which is very suitable for the production of mRNA for in vivo therapeutic purposes. The improvements in the vector in particular reside in the presence of a transcription enhancer and a nuclear retention element.

公开(公告)号：EP3068888B1

公开(公告)日：2018-01-31

申请号：EP14799996.5

申请日：2014-11-12

申请人： Vrije Universiteit Brussel

发明人： HEIRMAN, Carlo ,  THIELEMANS, Kristiaan

IPC分类号： C12N15/85 ,  A61K39/00 ,  A61K31/7088

CPC分类号： A61K48/0066 ,  A61K31/7105 ,  A61K39/0011 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/55516 ,  C07K14/70575 ,  C07K14/70596 ,  C12N15/85 ,  C12N2320/52 ,  C12N2830/50 ,  C12N2840/105

摘要： The present invention in general relates to an improved RNA transcription vector, which is very suitable for the production of mRNA for in vivo therapeutic purposes. The improvements in the vector in particular reside in the presence of a transcription enhancer and a nuclear retention element.

公开(公告)号：EP3068888A1

公开(公告)日：2016-09-21

申请号：EP14799996.5

申请日：2014-11-12

申请人： Vrije Universiteit Brussel

发明人： HEIRMAN, Carlo ,  THIELEMANS, Kristiaan

IPC分类号： C12N15/85 ,  A61K39/00 ,  A61K31/7088

CPC分类号： A61K48/0066 ,  A61K31/7105 ,  A61K39/0011 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/55516 ,  C07K14/70575 ,  C07K14/70596 ,  C12N15/85 ,  C12N2320/52 ,  C12N2830/50 ,  C12N2840/105

摘要： The present invention relates to an improved RNA transcription vector, which is very suitable for the production of mRNA for in vivo therapeutic purposes. The improvements in the vector reside in the presence of a translation enhancer (TE) and a nuclear retention element (NRS), especially when the latter is the “Expression and Nuclear Retention Element” (ENE) of Kaposi's sarcoma associated Herpes virus (KSHV).

摘要翻译： 本发明涉及一种改进的RNA转录载体，其非常适合用于体内治疗目的的mRNA的产生。 载体中的改进位于翻译增强子（TE）和核保留元件（NRS）的存在下，特别是当后者是卡波西肉瘤相关疱疹病毒（KSHV）的“表达和核保留元件”（ENE）时， 。