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    Labeling reactants and their use
    1.
    发明公开
    Labeling reactants and their use 有权
    Markierungsreagenzien und deren Verwendung

    公开(公告)号：EP0967205A1

    公开(公告)日：1999-12-29

    申请号：EP99660100.1

    申请日：1999-06-03

    申请人： WALLAC OY

    发明人： Takalo, Harri ,  Hovinen, Jari ,  Mukkala, Veli-Matti ,  Liitti, Päivi ,  Mikola, Heikki

    IPC分类号： C07D213/38 ,  C07D401/14 ,  G01N33/48

    CPC分类号： C07D401/14 ,  C07D213/38 ,  G01N33/533 ,  Y02P20/55

    摘要： The invention relates to a novel labeling reactant, suitable for labeling of a biospecific binding reactant using solid-phase synthesis. The invention further concerns new labeling methods. The novel labeling reactant has the formula (I)
    wherein

    A is a bivalent aromatic structure capable of absorbing light or energy and transferring the excitation energy to a lanthanide ion after the product made by the said solid-phase synthesis has been released from the used solid support, deprotected and converted to a lanthanide chelate;
    R is - Z(G' - NH - X) G" - E, where
    X is a transient protecting group;
    E is a carboxylic acid, its salt, active ester or halide;
    Z is the bridge point;
    G is a bridge between A and Z, G' is a bridge between NH and Z and G" is a bridge between E and Z.
    R' is -COOR''' where R''' is an alkyl of 1 to 4 carbon atoms, phenyl or benzyl.

    摘要翻译： 本发明涉及适用于使用固相合成标记生物特异性结合反应物的新型标记反应物。 本发明还涉及新的标记方法。 新型标记反应物具有式（I）其中A是能够吸收光或能量的二价芳族结构，并且在通过所述固相合成制成的产物已被释放后，将激发能转移到镧系元素离子 使用的固体支持物，去保护并转化成镧系螯合物; R是-Z（G'-NH-X）G“-E，其中X是瞬时保护基; E是羧酸，其盐，活性酯或卤化物; Z是桥点; G是 A和Z，G'是NH和Z之间的桥，G“是E和Z之间的桥.R'是-COOR”，其中R“是1至4个碳原子的烷基，苯基或苄基 。