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    Microchip large volume polymerase chain reaction (PCR) with integrated real-time capillary electrophoresis detection
    2.
    发明公开
    Microchip large volume polymerase chain reaction (PCR) with integrated real-time capillary electrophoresis detection 有权
    Mikrochipfürdie Polymerase-Kettenreaktion（PCR）bei grossen Volumen mit Detektion per Kapillarelektrophorese in Echtzeit

    公开(公告)号：EP2717041A1

    公开(公告)日：2014-04-09

    申请号：EP14150172.6

    申请日：2008-06-11

    申请人： Wako Pure Chemical Industries, Ltd.

    发明人： Bousse, Luc ,  Zhang, Jian-ping

    IPC分类号： G01N27/447 ,  C12P19/34 ,  G01N30/00 ,  C12Q1/68 ,  B01L3/00

    CPC分类号： G01N27/44791 ,  B01L3/502746 ,  B01L3/502753 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2400/0421 ,  G01N27/44721

    摘要： A microfluidic device is provided with appropriate integrated structures to carry out large volume PCR and end-point or real-time capillary electrophoresis detection. The microfluidic device includes a substrate having a chamber for amplification of a volume of nucleic acid; wells disposed on the substrate; flow channels connecting the wells and the chamber in the substrate to allow for flow of solution through the chamber; and one or more separation channels provided in the substrate and connected to the chamber for separating and detecting a fraction of the amplified nucleic acid in the chamber. The chamber, the flow channels, and the one or more separation channels are configured such that the hydrodynamic flow resistance of the chambers and the flow channels combined is at least 10 3 times smaller than the hydrodynamic flow resistance in the one or more separation channels. The microfluidic device can achieve a very high sensitivity in detection while being highly cost effective.

    摘要翻译： 微流体装置具有适当的整合结构，可进行大容量PCR和终点或实时毛细管电泳检测。 微流体装置包括具有用于扩增一定体积的核酸的室的底物; 设置在基板上的孔; 将孔和腔室连接在衬底中以允许溶液流过腔室的流动通道; 以及一个或多个分离通道，其设置在所述基底中并连接到所述腔室，用于分离和检测所述腔室中所述扩增的核酸的一部分。 腔室，流动通道和一个或多个分离通道被构造成使得组合的腔室和流动通道的流体动力学流动阻力比一个或多个分离通道中的流体动力流动阻力小至少10 3倍。 微流体装置可以在检测中获得非常高的灵敏度，同时具有高成本效益。