公开(公告)号：EP2207901B1

公开(公告)日：2018-07-25

申请号：EP08839468.9

申请日：2008-10-17

申请人： Wisconsin Alumni Research Foundation

发明人： SCHWARTZ, David, C. ,  POTAMOUSIS, Konstantinos, D. ,  ZHOU, Shiguo ,  GOLDSTEIN, Steven, J. ,  NEWTON, Michael, A. ,  RUNNHEIM, Rodney, A. ,  FORREST, Daniel, K. ,  CHURAS, Christopher, P.

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6869 ,  C12Q2535/101

摘要： Methods are provided for ascertaining the sequence of a genomic DNA sample by nicking the DNA sample with a restriction nicking enzyme, followed by nick translation with labeled nucleotides, such that the labeled nucleotides can be quantified and compared to a known, reference genome.

公开(公告)号：EP2207901A1

公开(公告)日：2010-07-21

申请号：EP08839468.9

申请日：2008-10-17

申请人： WISCONSIN ALUMNI RESEARCH FOUNDATION

发明人： SCHWARTZ, David, C. ,  POTAMOUSIS, Konstantinos, D. ,  ZHOU, Shiguo ,  GOLDSTEIN, Steven, J. ,  NEWTON, Michael, A. ,  RUNNHEIM, Rodney, A. ,  FORREST, Daniel, K. ,  CHURAS, Christopher, P.

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6869 ,  C12Q2535/101

摘要： Methods are provided for ascertaining the sequence of a genomic DNA sample by nicking the DNA sample with a restriction nicking enzyme, followed by nick translation with labeled nucleotides, such that the labeled nucleotides can be quantified and compared to a known, reference genome.

摘要翻译： 提供了通过用限制性切割酶切割DNA样品，随后用标记的核苷酸进行切口平移来确定基因组DNA样品的序列的方法，使得标记的核苷酸可以被定量并与已知的参考基因组进行比较。