公开(公告)号：EP3350324A1

公开(公告)日：2018-07-25

申请号：EP16846943.5

申请日：2016-09-13

申请人： Wroclawskie Centrum Badan EIT + SP Z O.O.

发明人： LIPINSKI, Tomasz ,  SKOWICKI, Michal ,  BEDNARKIEWICZ, Artur

IPC分类号： C12N13/00 ,  G01N21/64

CPC分类号： G01N21/6428 ,  C07K16/00 ,  C07K2317/14 ,  G01N21/6458 ,  G01N21/6489 ,  G01N2021/6441

摘要： The object of the invention is the method of detection and selection of hybridoma cells capable to produce desired antibodies, comprising seeding the hybridoma cells in a culture vessel with biofunctionalized surface, containing culture medium, adding biofunctionalized luminescent labels and incubating such hybridoma cell culture, followed by optical detection of hybridoma cells producing desired antibodies by reaction of he biofunctionalized luminescent labels with the antibodies and detecting a luminescent label's signal border around hybridoma cells producing desired antibodies, and further separation in situ hybridoma cells producing the given antibody type from the rest of the cells.