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公开(公告)号：EP0994969B1
公开(公告)日：2006-04-05
申请号：EP98932421.5
申请日：1998-07-13
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q2565/518 , C12Q2563/131 , C12Q2531/119
摘要： Provided is a method for characterising one or more nucleic acids, which method comprises immobilising double-stranded nucleic acids on a solid phase support, cleaving the immobilised nucleic acids with an endonuclease such that each cleaved nucleic acid has a double-stranded portion, denaturing the cleaved nucleic acids to form single-stranded cleaved nucleic acid, hybridising one or more oligonucleotide sequences to the resulting single-stranded cleaved nucleic acid, each oligonucleotide sequence comprising a pre-determined recognition sequence situated such that it recognises a sequence which was part of the double-stranded portion of the nucleic acid and a label specific to the recognition sequence, extending correctly hybridised oligonucleotide sequences along the single-stranded portion of the immobilised nucleic acid to form an extended strand, denaturing the extended strand from the immobilised strand and characterising the immobilised nucleic acid by identifying the size of the extended strand and the identity of the recognition sequence.
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公开(公告)号：EP1095053B1
公开(公告)日：2002-12-11
申请号：EP99933033.5
申请日：1999-07-13
CPC分类号： C07H21/00 , C12Q1/6872
摘要： Provided is a method for characterising an analyte, which method comprises: (a) providing a compound in which the analyte is attached by a cleavable linker to a reporter group relatable to the analyte, the compound having formula (I) wherein either R comprises the reporter group and R' comprises the analyte, or R comprises the analyte and R' comprises the reporter group, and wherein n is 1 or 2; (b) cleaving the reporter group from the analyte; and (c) identifying the reporter group, thereby characterising the analyte.
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公开(公告)号：EP1275004A2
公开(公告)日：2003-01-15
申请号：EP01911912.2
申请日：2001-03-14
CPC分类号： C07H21/00 , C07K1/1077 , C12Q1/6872 , C40B40/00 , G01N33/6803 , G01N2458/15 , Y10T436/13
摘要： Provided is a set of two or more mass labels, each label in the set comprising a mass marker moiety attached via a cleavable linker to a mass normalisation moiety, the mass marker moiety being fragmentation resistant, wherein the aggregate mass of each label in the set may be the same or different and the mass of the mass marker moiety of each label in the set may be the same or different, and wherein in any group of labels within the set having a mass marker moiety of a common mass each label has an aggregate mass different from all other labels in that group, and wherein in any group of labels within the set having a common aggregate mass each label has a mass marker moiety having a mass different from that of all other mass marker moieties in that group, such that all of the mass labels in the set are distinguishable from each other by mass spectrometry.
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