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1.发明公开Method of enzymatically synthesizing 3'-phosphoadenosine-5'-phophosulfate 审中-公开维生素Zur酶联免疫球蛋白3'-Phosphoadenosin-5'-磷磷酸
公开(公告)号：EP2100956A1
公开(公告)日：2009-09-16
申请号：EP09004307.6
申请日：2006-01-24
申请人： YAMASA CORPORATION
发明人： Ishige, Kazuya , Noguchi, Toshitada
CPC分类号： C12N9/1229 , C12N9/1205 , C12N9/1241 , C12P19/32
摘要： A DNA fragment comprising a polynucleotide which is formed of nucleotides 101 to 1,171 in a nucleotide sequence of SEQ ID NO: 9, and which encodes polyphosphate-driven nucleoside 5'-diphosphate kinase (PNDK), wherein a nucleotide sequence corresponding to at least 72 N-terminal amino acid residues is deleted.
摘要翻译： 一种DNA片段，其包含由SEQ ID NO：9的核苷酸序列中的核苷酸101-1,171形成并且编码多磷酸盐驱动的核苷5'-二磷酸激酶（PNDK）的多核苷酸，其中对应于至少72 缺失N-末端氨基酸残基。
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公开(公告)号：EP2100956B1
公开(公告)日：2018-11-21
申请号：EP09004307.6
申请日：2006-01-24
申请人： YAMASA CORPORATION
发明人： Ishige, Kazuya , Noguchi, Toshitada
CPC分类号： C12N9/1229 , C12N9/1205 , C12N9/1241 , C12P19/32
摘要： The invention provides a method for producing 3'-phosphoadenosine 5'-phosphosulfate (PAPS), the method including subjecting ATP to sulfation and phosphorylation by use of adenosine 5'-triphosphate sulfurylase (ATPS) and adenosine 5'-phosphosulfate kinase (APSK), wherein an adenosine 5'-triphosphate (ATP) supply/regeneration system including adenosine 5'-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5'-diphosphate kinase (PNDK), and polyphosphate:AMP phosphotransferase (PAP), or an adenosine 5'-triphosphate (ATP) supply/regeneration system including adenosine 5'-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5'-diphosphate kinase (PNDK), and adenylate kinase (ADK) is employed instead of ATP.
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