公开(公告)号：EP3119902A4

公开(公告)日：2017-11-29

申请号：EP15765747

申请日：2015-03-18

申请人： APTEVO RES AND DEV LLC

发明人： SEWELL ELAINE ,  BLANKENSHIP JOHN ,  LOWE RICHARD

IPC分类号： C12P21/08 ,  A01N63/00 ,  A61K39/00 ,  A61K39/395 ,  C07K16/00 ,  C12N5/07 ,  G01N33/50

CPC分类号： G01N33/5011 ,  A61K2039/505 ,  C07K16/28 ,  C07K16/2803 ,  C07K16/2809 ,  C07K16/2896 ,  C07K16/3069 ,  C07K16/32 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/622 ,  C07K2317/64 ,  C07K2317/732 ,  G01N33/5014 ,  G01N33/505 ,  G01N2333/7051 ,  G01N2333/70596 ,  G01N2500/02 ,  G01N2500/10 ,  G01N2510/00

摘要： This disclosure provides a cell-based assay for testing the potency of multispecific binding molecules which specifically bind a T-cell antigen and a target antigen for redirected T-cell-mediated cellular cytotoxicity. The assay uses the TALL-104 T-cell line as effector cells, and provides a sensitive, specific, and reproducible method for ensuring that purity, activity, and stability of multispecific binding molecule batches can be measured for development, clinical trials, and commercial marketing.