公开(公告)号：EP3084436A4

公开(公告)日：2017-05-24

申请号：EP14872357

申请日：2014-12-17

申请人： BOARD OF TRUSTEES SOUTHERN ILLINOIS UNIV

发明人： HALFORD WILLIAM P

IPC分类号： G01N33/53

CPC分类号： G01N33/56994 ,  G01N2333/035 ,  G01N2469/20

摘要： An assay for infection with one or more herpes simplex viruses that comprises a) dividing an antibody-containing serum sample into at least three serum subsamples, and b) separately incubating each with a physical matrix of cell antigens from i) uninfected cells, (ii) HSV-1-infected cells, or (iii) HSV-2-infected cells to form at least three serum subsample admixtures so that antibodies present in each admixture can immunoreact to form matrix-bound antibodies and at least three preadsorbed serum subsample portions. Each preadsorbed serum subsample portion is incubated with a mixture of matrix-bound antigens from cells uninfected by HSV-1 or HSV-2, infected by each of HSV-1 and by HSV-2 to permit antibodies to immunoreact with antigens present to form three matrix-bound immunoreactants. The amount of each immunoreaction is determined, and reaction amounts are prognostic for whether the subject whose serum was tested is infected by one, both, or neither of HSV-1 and HSV-2.

摘要翻译： 一种用一种或多种单纯疱疹病毒感染的测定，其包括a）将含有抗体的血清样品分成至少三个血清子样品，和b）分别与来自i）未感染细胞的细胞抗原的物理基质孵育，（ii） ）HSV-1感染的细胞或（iii）HSV-2感染的细胞以形成至少三种血清亚样品混合物，使得存在于每种混合物中的抗体可以免疫反应以形成基质结合的抗体和至少三种预先吸附的血清亚样品部分。 将每个预先吸附的血清子样品部分与来自HSV-1或HSV-2感染的细胞的基质结合抗原的混合物一起孵育，所述HSV-1或HSV-2感染HSV-1和HSV-2中的每一种，以允许抗体与存在的抗原免疫反应以形成三 基质结合的免疫反应物。 确定每种免疫反应的量，并且反应量对于其血清被测试的受试者是否感染HSV-1和HSV-2中的一种，两者或两者都是预后的。