公开(公告)号：EP4431609A1

公开(公告)日：2024-09-18

申请号：EP23161904.0

申请日：2023-03-14

申请人： Adisseo France S.A.S. ,  Institut National des Sciences Appliquées de Toulouse ,  INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT ,  Centre national de la recherche scientifique

发明人： MALFOY, Thibault ,  BARTHE, Manon ,  AURIOL, Clément ,  FRANCOIS, Jean Marie

IPC分类号： C12P7/52 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88

CPC分类号： C12P7/52 ,  C12Y101/01027 ,  C12Y101/01028 ,  C12Y101/01037 ,  C12Y101/01082 ,  C12Y101/01299 ,  C12Y206/01001 ,  C12Y206/01042 ,  C12Y206/01057 ,  C12Y401/02028 ,  C12R2001/1920210501 ,  C12N9/0006 ,  C12N9/1096 ,  C12N9/88 ,  C12N9/0008

摘要： The present invention relates to an improved method for the preparation of 2,4-dihydroxybutyrate from a carbon source via homoserine comprising a two steps pathway:
- a first step of conversion of the primary amino group of homoserine to a carbonyl group to obtain 2-oxo-4-hydroxybutyrate catalysed by an enzyme having homoserine transaminase activity as defined by E.C. 2.6.1.1 or E.C. 2.6.1.42 or E.C. 2.6.1.57 and
- a second step of reduction of the obtained 2-oxo-4-hydroxybutyrate (OHB) to 2,4-dihydroxybutyrate catalysed by an enzyme having OHB reductase activity, the enzyme having OHB reductase activity is lactate dehydrogenase as defined by E.C.1.1.1.27 or E.C.1.1.1.28, or malate dehydrogenase as defined by E.C.1.1.1.37, E.C.1.1.1.82 or E.C.1.1.1.299, and
wherein at least one enzyme having aldolase activity as defined by E.C.4.1.2.28 is inactivated to prevent OHB degradation.