公开(公告)号：EP4461129A1

公开(公告)日：2024-11-13

申请号：EP23172320.6

申请日：2023-05-09

申请人： ZHAW - Zürcher Hochschule für Angewandte Wissenschaften

发明人： EIBL, Regine ,  EIBL, Dieter ,  HÜHN, Tilo ,  MOZAFFARI, Fruhar

IPC分类号： A01H4/00 ,  C12P7/22 ,  A01H5/08 ,  A01H6/52

摘要： A method of processing plant cell cultures based on fruit explants is described, comprising the steps of: a) inducing callus from an explant of a fruit selected from drupes, nuts, legumes or berries on callus induction medium and propagating the callus on callus growth medium; b) selecting a callus clone from the grown callus culture and initiating a suspension cell culture comprising dedifferentiated cells by inoculating the selected callus clone into a suspension cell culture medium; c) growing fruit suspension cells for a time and under conditions sufficient to produce a suspension including a biomass comprising secondary metabolites; and d) separating the biomass from the suspension to provide a liquid phase and a solid, in vitro-manufactured biomass and optionally extracting secondary metabolites from the in vitro -manufactured biomass to provide a secondary metabolite extract, wherein the fruit is selected from and the explant contains endocarp and/or seed material from the drupes, nuts, legumes or berries. The method enables biomass to be manufactured in vitro at improved efficiencies and yields. In addition, in vitro-manufactured biomass prepared by the aforementioned method, uses of the same, and related food compositions are described.