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    METHOD FOR PRODUCING FIBRIN SHEET
    3.
    发明公开
    METHOD FOR PRODUCING FIBRIN SHEET 审中-公开

    公开(公告)号：EP4039701A1

    公开(公告)日：2022-08-10

    申请号：EP20870789.3

    申请日：2020-09-09

    申请人： OSAKA UNIVERSITY

    发明人： SAMURA, Takaaki ,  MIYAGAWA, Shigeru ,  SAWA, Yoshiki ,  YOKOYAMA, Junya

    IPC分类号： C07K14/75 ,  C07K14/78 ,  C12M3/00 ,  C12N5/071 ,  C12N9/74

    摘要： A method for producing a fibrin sheet containing at least one selected from the group consisting of cells and drugs in a fibrin gel, the method comprising:
    a step 1 of applying a fibrinogen solution containing at least one selected from the group consisting of cells and drugs and fibrinogen dropwise onto a surface of a substrate made of a gelatin hydrogel;
    a step 2 of adding thrombin to the fibrinogen solution on the surface of the substrate;
    a step 3 of placing a support film on and in contact with a top surface of the fibrinogen solution to which the thrombin has been added;
    a step 4 of forming a fibrin sheet containing the at least one selected from the group consisting of cells and drugs in a fibrin gel between the substrate and the support film by a reaction between the fibrinogen and the thrombin; and
    a step 5 of melting the substrate at a temperature not lower than a melting temperature of the gelatin hydrogel to separate, from the substrate, the fibrin sheet supported by the support film.

    METHOD FOR PURIFYING FIBRINOGEN
    7.
    发明公开
    METHOD FOR PURIFYING FIBRINOGEN 审中-公开

    公开(公告)号：EP3404037A1

    公开(公告)日：2018-11-21

    申请号：EP17738641.4

    申请日：2017-01-12

    申请人： Green Cross Holdings Corporation

    发明人： KIM, Jun Sic ,  KIM, Hyo Jin ,  PARK, Ji Yoon ,  LEE, Ju Ho ,  SON, Jae Woon ,  SHIN, Yong Won

    IPC分类号： C07K1/30 ,  C07K1/34 ,  C07K1/36 ,  B01J20/08 ,  C07K14/75 ,  A61K38/36

    CPC分类号： C07K14/75 ,  A61K38/36 ,  B01J20/08 ,  C07K1/30 ,  C07K1/34 ,  C07K1/36

    摘要： The present disclosure relates to a method for purifying fibrinogen, and more particularly to a method for purifying fibrinogen, comprising the steps of: (a) precipitating fibrinogen of a fibrinogen-containing solution by adding glycine to the solution for a concentration of glycine to be 1.5 to 2.5M, and then removing a supernatant and recovering a precipitate (1 st glycine precipitation); (b) dissolving the precipitate of 1 st glycine precipitation of step (a) in a dissolution buffer to obtain a solution, precipitating the solution by adding glycine thereto for a concentration of glycine to be 0.2 to 1.2M, and recovering a supernatant (2 nd glycine precipitation); (c) precipitating the supernatant of step (b) by adding glycine thereto for a concentration of glycine to be 1.5 to 2.5M, and recovering a precipitate (3 rd glycine precipitation); and (d) dissolving the precipitate of step (c) in a dissolution buffer to obtain a solution, and subjecting the solution to nanofiltration (NF) using a nanofilter.
    The novel method for purifying fibrinogen protein according to the present disclosure is a highly safe method that fundamentally blocks the entry of viruses. Furthermore, it can purify fibrinogen protein with high purity and high recovery rate by a simple process, and thus is very economical and efficient. In addition, fibrinogen purified by the method of the present disclosure has an advantage over fibrinogen purified by a conventional method in that it has higher purity, and thus has increased local activity, indicating that it can be effectively used for the prevention and treatment of blood-related diseases, particularly blood clotting diseases.