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    Process and device for separating electrically charged macromolecular compounds by forced-flow membrane electrophoresis
    2.
    发明公开
    Process and device for separating electrically charged macromolecular compounds by forced-flow membrane electrophoresis 失效
    方法和装置用于通过强制流动膜电泳的手段分离带电大分子化合物。

    公开(公告)号：EP0369945A2

    公开(公告)日：1990-05-23

    申请号：EP89810861.8

    申请日：1989-11-09

    申请人： ALIGENA AG

    发明人： Perry, Mordechai, Dr. ,  Gilron, Jack, Dr. ,  Ketraro, Reuven ,  Linder, Charles, Dr.

    IPC分类号： B01D57/02 ,  B01D61/42 ,  G01N27/447 ,  C07K3/14

    CPC分类号： G01N27/44769 ,  B01D57/02 ,  C07K1/24 ,  C07K1/26

    摘要： A process and apparatus for the continuous separation of electrically charged macromolecular compounds, such as proteins, by forced-flow membrane electrophoresis is provided.
    Said process is conducted in an electrophoretic cell comprising a membrane compartment, divided by a micro- or ultrafiltration membrane (1) into a feed (2) and permeate cell (3), and electrode compartments (6, 7) being partitioned from the membrane compartment with ion-exchange membranes (8, 9), which are impermeable to the macromolecular compounds, and have virtually no permeability to salts.
    The electrophoretic process comprises the steps of recycling a mixed solution of the charged compounds which are dissolved in a separation buffer through the feed cell, subjecting of the solution to a pressure-­driven membrane separation, applying an electric field across the membranes and the solution to cause electrophoretic migration of the charged compound and to separate them, and withdrawing the charged compounds thus separated.
    The process achieves separation of charged compounds having differences of their isoelectric points in a range of as low as 0.1 to 0.2. The process is very useful to remove charged contaminants from the desired compounds.

    摘要翻译： 一种用于电的连续分离的方法和装置带电大分子化合物，颜色：例如蛋白质，通过提供强制流动电泳膜。 所述方法的实施在一个电泳电池，其包括膜隔室，通过一个微或超滤膜（1）划分为进料（2）和渗透细胞（3），和电极室（6,7）被从膜分配 隔室用离子交换膜（8,9），它们是不可渗透大分子化合物，和几乎没有渗透性的盐。 电泳方法包括回收其在通过所述进料单元的分离缓冲液中溶解的带电化合物的混合溶液中，该溶液的压力驱动的膜分离进行，横跨膜和溶液施加到电场的步骤 使带电化合物的电泳迁移和将它们分开，并回抽由此分离的电荷的化合物。 该过程实现了具有范围低至0.1至0.2的等电点的差异带电化合物的分离。 该过程是非常有用的，以除去从被充电所需化合物的污染物。

    Pre-cast gel systems for two-dimensional electrophoresis
    4.
    发明公开
    Pre-cast gel systems for two-dimensional electrophoresis 失效
    用于二维电泳的预凝胶系统

    公开(公告)号：EP0366897A3

    公开(公告)日：1991-10-23

    申请号：EP89116029.3

    申请日：1989-08-30

    申请人： BIO-RAD LABORATORIES, INC.

    发明人： Hochstrasser, Denis Francois

    IPC分类号： G01N27/26 ,  C07K3/14

    CPC分类号： G01N27/44773 ,  G01N27/44704

    摘要： A strip gel and a slab gel are combined in a single gel enclosure or on a single gel backing for two-dimensional electrophoresis. The enclosure is comprised of a single pair of support plates, and a removable electrically insulating region is included to separate the gels. The region is occupied either by air or an electrically nonconductive material which can be removed without disturbing either gel. In use, a sample is loaded onto one end of the strip gel and an electric potential is imposed across the strip gel for the first dimension separation while the strip gel is electrically insulated from the slab gel. The insulating region is then removed and the two gels placed in electrical contact for the second dimension separation.

    Procédé et dispositif d'électrophorèse multiple pour assurer la migration contrôlée de macromolécules dans des plaques de gel
    6.
    发明公开
    Procédé et dispositif d'électrophorèse multiple pour assurer la migration contrôlée de macromolécules dans des plaques de gel 失效
    在凝胶板大分子的多个电泳受控迁移的方法和装置。

    公开(公告)号：EP0358556A1

    公开(公告)日：1990-03-14

    申请号：EP89402399.3

    申请日：1989-09-04

    申请人： BERTIN & CIE

    发明人： Dutertre, Bernard

    IPC分类号： B01D57/02 ,  C07K3/14 ,  G01N27/26

    CPC分类号： B01D57/02 ,  C07K1/26 ,  G01N27/4473 ,  G01N27/44773

    摘要： Procédé et dispositif d'électrophorèse multiple pour la migration contrôlée de macromolécules dans des plaques de gel empilées dans une direction perpendiculaire à leur plan, au moyen d'une série d'électrodes (20) disposées selon les intersections de deux séries de plans perpendicu­laires, les uns perpendiculaires aux plaques (12) et à la di­rection souhaitée de migration des macromolécules, les autres perpendiculaires aux premiers et parallèles aux plaques (12) , les électrodes étant reliées entre elles de façon sélective et commandées pour assurer, d'une part, la séparation des macro­molécules dans les plaques (12), puis, d'autre part, leur transfert sur des membranes (22) associées aux plaques (12).
    L'invention s'applique notamment à la séparation et au transfert par électrophorèse de macromolécules telles que des protéines ou des acides nucléiques.

    Highly purified human antihemophilic factor and method for the preparation thereof
    8.
    发明公开
    Highly purified human antihemophilic factor and method for the preparation thereof 失效
    高度纯化的人抗血友病因子和方法及其生产。

    公开(公告)号：EP0104356A1

    公开(公告)日：1984-04-04

    申请号：EP83107420.8

    申请日：1983-07-28

    申请人： THE UNIVERSITY OF ROCHESTER

    发明人： Chavin, Stephen I. ,  Fay, Philip J.

    IPC分类号： C07K3/14 ,  C07K3/20 ,  C07K3/22 ,  A61K35/16

    CPC分类号： C07K14/755 ,  A61K38/00 ,  Y10S530/83

    摘要： Highly purified, biologically active Human Antihemophilic Factor (AHF) preparations are prepared having specific activities of about 4000 - 8000 units per milligram of AHF. In the method of preparation an AHF concentrate, prepared by fractionation of plasma to partially remove fibrinogen, fibronectin and other plasma components is subjected to a separation on the basis of Stokes' radius to separate AHF from the bulk of remaining proteins in the AHF concentrate. The pooled fractions containing AHF activity are concentrated by precipitation with ammonium sulfate, sodium sulfate, etc., by diafiltration, by PEG addition, or the like. The concentrate, is solubilized or equilibrated in an aqueous medium and treated to change the effective Stokes' radius of the AHF to an apparently low value and then subjected to a separation from the concentrate. The AHF pool from above is treated to remove cations by dialysis against an appropriate buffer of lower ionic strength and chromatographed on an anion-exchange medium. The AHF fraction from the above chromatography, is a highly purified AHF preparation.