利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

52 条记录 (耗时 0.014 秒)

    Process for producing a solid phase immunoassay
    2.
    发明公开
    Process for producing a solid phase immunoassay 失效
    Verfahren zur Herstellung eines Fest-Phasen-Immunoassays。

    公开(公告)号：EP0115681A1

    公开(公告)日：1984-08-15

    申请号：EP83307533.6

    申请日：1983-12-12

    申请人： WARNER-LAMBERT COMPANY

    发明人： Barnes, Wayne Riley ,  Johnson, Teresa

    IPC分类号： G01N33/54 ,  G01N33/56

    CPC分类号： G01N33/545

    摘要： There is disclosed a process for preparing a solid phase immunoassay, which process comprises:

    purifying a specific binding protein;
    reacting the purified specific binding protein with one or more aldehyde to enhance the hydrophobic nature of the protein without crosslinking or polymerizing the protein;
    contacting the surface of an insoluble support with the enhanced hydrophobic specific binding protein in the presence of a buffer and bonding the specific binding protein to the surface; and
    removing any remaining unbound protein from the insoluble support.

    An associated method and kit for assaying a specimen for a specific component of the specimen are also disclosed. The invention enables the protein to be bound to the insoluble support more securely than in the absence of the aldehyde.

    摘要翻译： 公开了一种制备固相免疫测定法的方法，该方法包括：纯化特异性结合蛋白; 使纯化的特异性结合蛋白与一种或多种醛反应，以增强蛋白质的疏水性，而不使蛋白质交联或聚合; 在缓冲液存在下使不溶性载体的表面与增强的疏水特异性结合蛋白接触，并将特异性结合蛋白结合到表面上; 并从不溶性支持物中除去任何剩余的未结合的蛋白质。 还公开了用于测定样品的特定组分的样品的相关方法和试剂盒。 本发明使得蛋白质能够比不存在醛的情况下更可靠地与不溶性载体结合。

    Quantitative determination of adenosine
    3.
    发明公开
    Quantitative determination of adenosine 失效
    定量测定腺苷。

    公开(公告)号：EP0070033A1

    公开(公告)日：1983-01-19

    申请号：EP82106276.7

    申请日：1982-07-13

    申请人： Yamasa Shoyu Kabushiki Kaisha

    发明人： Sato, Tomokazu ,  Ui, Michio

    IPC分类号： G01N33/54 ,  G01N33/56

    CPC分类号： G01N33/537 ,  G01N33/5308 ,  Y10S436/815

    摘要： A method of quantitative determination of adenosine by means of competitive immunoassay based on a competitive antigen-antibody reaction. In the competitive antigen-antibody reaction, an antibody is used which is obtained from an animal which has been immunized by introduction thereto of an antigen which comprises a carrier protein bonded with 2'- and 3'- hydroxyls of the adenosine through dicarboxylic acid residues, and a labelled adenosine and 2',3'-diacyladenosine which has been produced by acylation of adenosine in the sample to be assayed or in a standard solution are caused to undergo competitive reaction for the antibody whereby it has been made possible to determine adenosine quantitatively in high sensitivity and in high accuracy.

    Radioimmunoassay of migration inhibitory factor
    5.
    发明公开
    Radioimmunoassay of migration inhibitory factor 失效
    放射免疫移动抑制因子。

    公开(公告)号：EP0020090A1

    公开(公告)日：1980-12-10

    申请号：EP80301692.2

    申请日：1980-05-22

    申请人： MONSANTO COMPANY

    发明人： Brandt, Eva Jane ,  Asculai, Samuel Simon

    IPC分类号： G01N33/56

    CPC分类号： G01N33/6863 ,  Y10S436/804 ,  Y10S436/815

    摘要： A method is disclosed for detecting the presence of migration inhibitory factor (MIF) which comprises reacting a test solution containing an unknown amount of MIF antigen, a known amount of MIF antigen which has been radiolabeled, a known amount of antibodies to MIF, and thereafter precipitating and removing the resulting immune complex and measuring the radioactive content thereof, said antibodies to MIF being produced by immunization of animals with MIF antigen obtained from the growth of human lymphocytes under tissue culture conditions.

    Antikörper gegen bakterielle Peptidoglycane, Verfahren zu ihrer Herstellung und Methoden zu ihrer quantitativen Bestimmung
    9.
    发明公开
    Antikörper gegen bakterielle Peptidoglycane, Verfahren zu ihrer Herstellung und Methoden zu ihrer quantitativen Bestimmung 失效
    针对细菌肽的抗体，它们的制备方法和它们的定量测定的方法流程。

    公开(公告)号：EP0101886A2

    公开(公告)日：1984-03-07

    申请号：EP83107087.5

    申请日：1983-07-20

    申请人： Boehringer Ingelheim International GmbH

    发明人： Kolb, Helmut, Prof. Dr. ,  Schleifer, Karl Heinz, Prof. Dr. ,  Seidl, Hans Peter, Dr. ,  Tympner, Klaus Dieter, Prof. Dr. ,  Weiss, Ludwig, Dr.

    IPC分类号： C07G7/00 ,  G01N33/68 ,  G01N33/56 ,  G01N33/54 ,  G01N33/58

    CPC分类号： G01N33/531 ,  G01N33/564 ,  G01N33/56911

    摘要： Antikörper aus der Klasse der Immunglobuline G gegen das Peptiduntereinheit-Pentapeptid L-Ala-D-Glu(L-Lys-D-Ala-D-Ala) des Peptidoglycans in biologischem Untersuchungsmaterial sowie gegen diese Peptidoglycane enthaltende bakterielle Zellwände, gekennzeichnet durch

    a) spezifische Bindung an Pentapeptide der allgemeinen Formel
    worin

    R ein Aminosäureanteil mit 1 - 3, vorzugsweise 3 Aminosäuren aus der Gruppe Gly, L-Ala, D-Glu, L-Lys und
    A, eine der Aminosäuren D-Ala, D-Ser, D-Val oder D-Leu bedeuten, oder

    b) spezifische Bindung an Pentapeptide der allgemeinen Formel 1 enthaltende isolierte bakterielle Zellwände oder
    c) spezifische Bindung an Pentapeptide der allgemeinen Formel I enthaltende grampositive Bakterien, Verfahren zu ihrer Herstellung, Verfahren zu ihrer quantitativen Bestimmung und Verwendung

    摘要翻译： 从类免疫球蛋白G ^针对肽亚单位五肽在生物测试材料肽聚糖的L-丙氨酸-D-GLU（L赖氨酸-D-ALA-D-ALA）的抗体，以及对含有论文肽聚糖细菌细胞壁， A1 - - D - 由通式R的五肽A）特异性结合，其特征在于丙氨酸（I）其中R darstellt至氨基酸具有1-3个，优选3酸部分，其选自氨基酸甘氨酸包含，L-丙氨酸， D-谷氨酸，L-Lys和A1 darstellt的氨基酸的D-丙氨酸，D-SER，D-缬氨酸或D-Leu或b）含有通式I或c的五肽分离的细菌细胞壁上的特定键合的一个 ）对革兰氏阳性菌含有通式I的五肽，其制备方法特异性结合，对它们的定量测定，并且它们的使用方法。