Microchip and microparticle fractional collection apparatus
    37.
    发明专利
    Microchip and microparticle fractional collection apparatus 有权
    MICROCHIP和MICROPARTICLE FRACTIONAL收集装置

    公开(公告)号:JP2012127922A

    公开(公告)日:2012-07-05

    申请号:JP2010282167

    申请日:2010-12-17

    IPC分类号: G01N15/14

    摘要: PROBLEM TO BE SOLVED: To provide a microchip and a microparticle fractional collection apparatus capable of stably fractionally collecting microparticles at a high speed without damaging the microparticles subject to collection.SOLUTION: A microchip includes: a sample liquid feed channel 11 permitting a sample liquid 2 containing microparticles to flow through; at least one pair of sheath liquid feed channels 12a and 12b configured to merge to the sample liquid feed channel 11 from both sides thereof for permitting a sheath liquid 3 to flow through surrounding the sample liquid 2; a merging channel 13 connected to the sample liquid feed channel 11 and the one pair of the sheath liquid feed channels 12a and 12b for permitting the sample liquid and the sheath liquid to merge and flow through the merging channel; and at least one pair of discharge channels 15a and 15b connected to the merging channel. The microchip further includes a vacuum suction unit 14 connected to the merging channel 13 for sucking and drawing in the microparticles subject to collection.

    摘要翻译: 要解决的问题:提供一种微芯片和微粒分数收集装置,其能够高速地稳定地分级收集微粒而不损害收集的微粒。 解决方案:微芯片包括:允许含有微粒的样品液体2流过的样品液体进料通道11; 至少一对鞘液供给通道12a和12b被构造成从其两侧与样品液体供给通道11合并,以允许鞘液3流过包围样品液体2; 连接到样品液体供给通道11的合流通道13和一对鞘液供给通道12a和12b,用于允许样品液体和鞘液合并并流过合流通道; 以及连接到合流通道的至少一对放电通道15a和15b。 微芯片还包括连接到合流通道13的真空抽吸单元14,用于吸收和拉伸待收集的微粒。 版权所有(C)2012,JPO&INPIT

    Sample concentrating device and method
    38.
    发明专利
    Sample concentrating device and method 审中-公开
    样品浓缩装置和方法

    公开(公告)号:JP2009274041A

    公开(公告)日:2009-11-26

    申请号:JP2008130083

    申请日:2008-05-16

    摘要: PROBLEM TO BE SOLVED: To provide a sample concentrating device which can concentrate particles to be detected to a high concentration. SOLUTION: The inner wall surface of a passage 14 is negatively charged, and positive ions 30 contained in a sample solution 28 in the passage 14 are adsorbed on the inner wall surface of the passage 14. When an electric field is generated between electrodes 18, 19 for concentration, negatively charged particles 29 in the sample solution 28 in a concentration area between the electrodes 18, 19 for concentration is forced in the reverse direction of the electric field and moved (electrophoresis), and attracted to the electrode 18 for concentration to be flocculated and to be concentrated at the electrode 18 for concentration. The positive ions 30 are moved in the electric field direction by Coulomb force to generate a flow (electroosmotic flow) of the sample solution 28. Consequently, the particles 29 are continuously supplied to the concentration area by the electroosmotic flow, and the supplied particles 29 are concentrated by the electrophoresis. COPYRIGHT: (C)2010,JPO&INPIT

    摘要翻译: 要解决的问题:提供可以将待检测的颗粒浓缩到高浓度的样品浓缩装置。 解决方案:通道14的内壁表面带负电,并且包含在通道14中的样品溶液28中的正离子30被吸附在通道14的内壁表面上。当在通道14的内壁表面之间产生电场时, 用于浓缩的电极18,19在用于浓缩的电极18,19之间的浓度区域中的样品溶液28中的带负电荷的颗粒29被迫在电场的相反方向上移动(电泳),并被吸引到电极18 用于浓缩絮凝并浓缩在电极18上用于浓缩。 正离子30通过库仑力在电场方向上移动以产生样品溶液28的流动(电渗流)。因此,通过电渗流将粒子29连续地供给到浓度区域,并且所提供的颗粒29 通过电泳浓缩。 版权所有(C)2010,JPO&INPIT