31.
    发明专利
    失效

    公开(公告)号:JPS5633074B2

    公开(公告)日:1981-07-31

    申请号:JP11441476

    申请日:1976-09-25

    IPC分类号: C12N1/00 C12N1/32 C12R1/01

    32.
    发明专利
    失效

    公开(公告)号:JPS5633073B2

    公开(公告)日:1981-07-31

    申请号:JP11908777

    申请日:1977-10-05

    IPC分类号: C12N1/32 C12R1/38

    33.
    发明专利
    失效

    公开(公告)号:JPS5632912B2

    公开(公告)日:1981-07-30

    申请号:JP3228077

    申请日:1977-03-25

    IPC分类号: C12N1/32 C12R1/38

    PREPARATION OF AGAR MEDIUM
    34.
    发明专利

    公开(公告)号:JPS5645191A

    公开(公告)日:1981-04-24

    申请号:JP12178479

    申请日:1979-09-21

    IPC分类号: C12N1/32 C12R1/01 C12R1/38

    摘要: PURPOSE:To improve the colony forming characteristic of methanol assimilable bacteria and the conservability of the bacteria, by sterilizing a culturing medium composition containing agar by steam under pressure without heating and melting, and pouring into incubation vessels while it is still fluid. CONSTITUTION:A culturing medium composition containing agar and

    DDAMINOACYLASE AND ITS PREPARATION
    36.
    发明专利

    公开(公告)号:JPS5542534A

    公开(公告)日:1980-03-25

    申请号:JP11532378

    申请日:1978-09-19

    申请人: SANRAKU OCEAN CO

    摘要: PURPOSE:To prepare D-aminoacylase by culturing facultative methanol-assimilating bacteria. CONSTITUTION:A strain selected from pseudomonas aminovorans NCIB 9039, Pseudomonas sp. 1158 (FERM-P No.4632) and Pseudomonas sp.617 (FERM-P No.4631) is cultured in a conventional medium at 10-45 deg.C and pH 4.0-9.0 to obtain the objective D-aminoacylase. The characteristics of the aminoacylase are as follows: (1) substrate specificity, hydrolyzes the acyl group of N-acyl-D-amino acid, and inactive to N-acyl-glucosamine or N-acyl-ethanolamine; (2) stability, keeps its activity at 50 deg.C, most stable at pH 6-7; (3) molecular weight, 100,000; (4) isoelectric point, pH=4.95; (5) elemental analysis, C 54.33%, H 7.19% and N 16.37%; (6) inhibitor, remarkably inhibited by p-chloro-mercury benzoic acid., etc.

    PREPARATION OF CULTURED BACTERIAL CELL

    公开(公告)号:JPS54135296A

    公开(公告)日:1979-10-20

    申请号:JP4130578

    申请日:1978-04-10

    申请人: SHOWA DENKO KK

    IPC分类号: C12N1/32 C12R1/38

    摘要: PURPOSE:To prepare cultured bacterial cell (bacterial protein) useful as foods and feeds, from methanol, by the use of specific methanol-assimilable bacteria. CONSTITUTION:Bacteria belonging to Pseudomonas genus and capable of assimilating methanol, e.g. Pseudomonas SD-21 (FERM-P No.4441) are cultured in a medium containing methanol as main carbon source (methanol concentration;

    39.
    发明专利
    失效

    公开(公告)号:JPS5419464B2

    公开(公告)日:1979-07-16

    申请号:JP5238573

    申请日:1973-05-11

    IPC分类号: C12N1/32 C12R1/78

    40.
    发明专利
    失效

    公开(公告)号:JPS5419463B2

    公开(公告)日:1979-07-16

    申请号:JP4741073

    申请日:1973-04-26

    IPC分类号: C12N1/32 C12R1/72