Abstract:
Ligase detection reaction is utilized to distinguish minority template in the presence of an excess of normal template with a thermostable ligase. This process can be carried out with a mutant ligase, thermostable ligase, or a modified oligonucleotide probe. This procedure is particularly useful for the detection of cancer-associated mutations. It has the advantage of providing a quantitative measure of the amount or ratio of minority template.
Abstract:
PROBLEM TO BE SOLVED: To provide ligand-immunogen conjugates to enhance immunization effects with combination of cytokines.SOLUTION: There is provided folate-fluorescein isothiocyanate represented by formula.
Abstract:
PROBLEM TO BE SOLVED: To distinguish a minority template in the presence of an excessive normal template.SOLUTION: Ligase detection reaction is utilized to distinguish a minority template in the presence of an excessive normal template with a thermostable ligase. This process can be carried out with a mutant ligase, a thermostable ligase, or a modified oligonucleotide probe. This procedure is particularly useful for the detection of cancer-associated mutations. It has the advantage of providing a quantitative measure of the amount or ratio of the minority template.
Abstract:
PROBLEM TO BE SOLVED: To provide a method for detecting minority target nucleotide sequences which differ from one or more majority target nucleotide sequences by change, insertion, delation or translocation of a base, in a sample containing both the sequences. SOLUTION: Ligase detection reaction is utilized to distinguish minority template in the presence of an excess of normal template with a thermostable ligase. This process can be carried out with a mutant ligase, thermostable ligase, or a modified oligonucleotide probe, and is particularly useful for the detection of cancer-associated mutations. It has the advantage of providing a measure of the amount and ratio of the minority template. COPYRIGHT: (C)2009,JPO&INPIT