롱 PCR이 가능한 DNA 중합효소 및 이의 유전자들
    10.
    发明公开
    롱 PCR이 가능한 DNA 중합효소 및 이의 유전자들 有权
    用于长PCR的DNA聚合酶及其基因

    公开(公告)号:KR1020130030799A

    公开(公告)日:2013-03-27

    申请号:KR1020137000005

    申请日:2010-05-14

    IPC分类号: C12N9/10 C12N15/54 C12N15/63

    CPC分类号: C12N9/1252

    摘要: PURPOSE: A DNA polymerase is provided to ensure superior performance in PCR of long chain DNA and to be applied in various fields. CONSTITUTION: A DNA polymerase has an amino acid sequence of sequence number 2. A DNA polymerase gene encodes an amino acid sequence of sequence number 2. The DNA polymerase gene is sequence number 1. A recombinant vector contains the DNA polymerase gene. A host cell is transformed by the recombinant vector. A method for preparing the polymerase comprises a step of inducing expression of the recombinant protein and isolating the polymerase.

    摘要翻译: 目的:提供DNA聚合酶,以确保长链DNA PCR的优异性能并应用于各种领域。 构成:DNA聚合酶具有序列号2的氨基酸序列.DNA聚合酶基因编码序列号2的氨基酸序列.DNA聚合酶基因是序列号1.重组载体含有DNA聚合酶基因。 宿主细胞被重组载体转化。 制备聚合酶的方法包括诱导重组蛋白表达并分离聚合酶的步骤。