公开(公告)号：KR1020010096318A

公开(公告)日：2001-11-07

申请号：KR1020000020396

申请日：2000-04-18

申请人： 현창기 ,  신현길 ,  주식회사 아미노젠

发明人： 현창기 ,  신현길 ,  주육현

IPC分类号： C07K1/16

CPC分类号： C07K1/16 ,  C07K1/12 ,  C07K1/34 ,  C07K14/47 ,  C12Y304/21014 ,  C12Y304/24

摘要： PURPOSE: The anticancer peptides produced from blood proteins of domestic animals and their producing method are provided, which peptides inhibit MNNG(N-methyl-N-nitro-N-nitrosoguanidine) from being damaged by interaction with cells or nuclei, so that such antigenic toxicity can prevent and treat cancers. CONSTITUTION: The method for producing the anticancer peptides from blood proteins of domestic animals comprises the steps of: collecting the blood from the domestic animals and treating them with antithrombin; centrifuging the blood to separate serum and blood corpuscle, and separating albumin and globulin by adding ethanol to the serum; removing stroma heme from the blood corpuscle, and centrifuging and precipitating it to separate globin; adding a protease to albumin, globulin and globin to hydrolyze them; and subjecting the hydrolysate to gel chromatography and ultracentrifugation to isolate the anticancer peptides, in which the protease is microbial serine protease or metalloprotease.

摘要翻译： 目的：提供由家兔血液蛋白产生的抗癌肽及其制备方法，该肽可抑制MNNG（N-甲基-N-硝基-N-亚硝基胍）与细胞或细胞核的相互作用而受损，从而使这种抗原性 毒性可以预防和治疗癌症。 构成：从家畜血液蛋白制备抗癌肽的方法包括以下步骤：从家畜中收集血液并用抗凝血素处理; 离心血液分离血清和血小板，并通过向血清中加入乙醇分离白蛋白和球蛋白; 从血球中除去基质血红素，离心分离珠球蛋白沉淀; 向白蛋白，球蛋白和珠蛋白中加入蛋白酶水解它们; 并对该水解产物进行凝胶层析和超速离心以分离其中蛋白酶为微生物丝氨酸蛋白酶或金属蛋白酶的抗癌肽。

公开(公告)号：KR1020020074746A

公开(公告)日：2002-10-04

申请号：KR1020010014706

申请日：2001-03-21

申请人： 주식회사 아미노젠 ,  주육현 ,  현창기

发明人： 주육현 ,  현창기 ,  박금주

IPC分类号： A61K38/00

CPC分类号： A61K38/012 ,  A23L33/18

摘要： PURPOSE: Provided are anticancer agents having peptides produced by hydrolysis of fibroin which is obtained by extracting silk thread or waste silk thread of silkworm cocoon as a main component. These peptides can be applied to medicine and functional food having anticancer activity, so that it can contribute to the development of medicines useful for prevention and treatment of cancer. CONSTITUTION: These anticancer agents contain peptides produced from silkworm cocoon, wherein the peptides are prepared by the following process consisting of: treating silk thread or waste silk thread of silkworm cocoon with 1 to 10% sodium carbonate or sodium bicarbonate to remove sericin, and treating the obtained fibroin with acid such as hydrochloric acid or oxalic acid or protease.

摘要翻译： 目的：提供通过丝心蛋白水解产生的肽，其通过以丝蚕丝或蚕茧的丝线作为主要成分提取获得的抗癌剂。 这些肽可以应用于具有抗癌活性的药物和功能性食品，从而有助于开发可用于预防和治疗癌症的药物。 构成：这些抗癌剂含有由蚕茧产生的肽，其中肽通过以下方法制备，其包括：用1至10％碳酸钠或碳酸氢钠处理丝茧丝或丝丝蚕丝以除去丝胶蛋白，并处理 所得的丝心蛋白与酸如盐酸或草酸或蛋白酶。

公开(公告)号：KR1020000012640A

公开(公告)日：2000-03-06

申请号：KR1019990058086

申请日：1999-12-15

申请人： 주식회사 아미노젠

发明人： 주육현

IPC分类号： A23L1/29

摘要： PURPOSE: The production of hormone power is provided to improve the immunity function, to effectively use to nutrition distribution, to promote metastasis of human body by protecting the human body from various harmful factors, to improve the immunity function. CONSTITUTION: The production method of hormone power has the steps of: adding 18 kinds of high quality silk amino acid, peptide such as essential amino acid in 20 kinds of colostrum milk, evergreen palm berry extract powder, wood pulp extract powder, coral calcium powder, vitamins, etc.; moisture measuring, iconography, heavy metals, colon bacillus examining; mixing each material uniformly using a blender; forming the material mixed uniformly through a deliver nozzle with a former; wrapping according to the wrapping standard.

摘要翻译： 目的：提供激素能量的产生，以提高免疫功能，有效利用营养分布，通过保护人体免受各种有害因素促进人体转移，提高免疫功能。 构成：激素功率的生产方法步骤如下：在20种初乳乳中加入18种优质丝氨基酸，必需氨基酸肽，常绿棕榈浆提取粉，木浆提取粉，珊瑚钙粉 ，维生素等; 水分测量，图像，重金属，大肠杆菌检查; 使用搅拌器均匀混合每种材料; 通过具有前者的输送喷嘴均匀地混合材料; 根据包装标准包装。

公开(公告)号：KR1020010079286A

公开(公告)日：2001-08-22

申请号：KR1020010040562

申请日：2001-07-03

申请人： 주식회사 아미노젠

发明人： 주육현

IPC分类号： A23J3/30

摘要： PURPOSE: A hydrochloric acid hydrolysis method for manufacturing silk amino acid and silk peptide by using silkworm is provided to improve immunity, protect a human body from a variety of harmful elements and promote metabolism and nutrition supply. CONSTITUTION: High-quality silkworm is cut into 3 parts after removing a pupa and washed with purified water. Water and NaHCO3 5% solution are mixed in a ratio of 10:1 and boiled at 100deg.C for 2 hours for a primary washing. NaHCO3 1% solution is mixed and boiled at 100deg.C for 1 hour for a secondary washing to eliminate Serisin and residual NaHCO3. The cut silkworm is dried by using a dryer and dissolved in 1.5N HCl at 100deg.C for 48 hours without pressure. The dissolved material is aged for 24 hours and cooled. The color of the material is black. The pH of the cooled material solution is 7 by adding NaOH and about 15% of refined salt is produced. The black material solution is decolored/deodorized by using active carbons and filtered by using a filter. The filtrate is desalted by using a desalting machine and decompression-concentrated. The concentrate is dried and pulverized to be powder by using lyophilization and spray-drying methods. The dried powder is packed in a stick or a bottle.

摘要翻译： 目的：提供通过使用蚕丝制造丝氨基酸和丝肽的盐酸水解方法，以提高免疫力，保护人体免受各种有害元素的影响，促进新陈代谢和营养供应。 构成：去除蛹后，将高质量的蚕丝切成3份，用纯水洗净。 将水和NaHCO 3 5％溶液以10:1的比例混合并在100℃下煮沸2小时进行一次洗涤。 将NaHCO 3 1％溶液在100℃下混合并煮沸1小时进行二次洗涤以除去丝氨酸和残留的NaHCO 3。 通过使用干燥器干燥切割的蚕，并在100℃下溶解在1.5N HCl中48小时而没有压力。 将溶解的材料老化24小时并冷却。 材料的颜色是黑色。 通过加入NaOH，冷却的物质溶液的pH为7，产生约15％的精制盐。 黑色材料溶液通过使用活性炭进行脱色/除臭，并使用过滤器进行过滤。 通过使用脱盐机将滤液脱盐并减压浓缩。 通过使用冻干和喷雾干燥方法将浓缩物干燥并粉碎成粉末。 将干燥的粉末装入棒或瓶中。

公开(公告)号：KR1020010079285A

公开(公告)日：2001-08-22

申请号：KR1020010040561

申请日：2001-07-03

申请人： 주식회사 아미노젠

发明人： 주육현

IPC分类号： A23J3/34

摘要： PURPOSE: An enzyme hydrolysis method for manufacturing silk amino acid and silk peptide by using silkworm is provided to distinctly reduce the discharge of contaminated materials and to prevent the accident in a working place. CONSTITUTION: High-quality silkworm is cut into 3 parts after removing a pupa and washed with purified water. Water and NaHCO3 5% solution are mixed in a ratio of 10:1 and boiled at 100deg.C for 2 hours for a primary washing. NaHCO3 1% solution is mixed and boiled at 100deg.C for 1 hour for a secondary washing to eliminate Serisin and residual NaHCO3. The cut silkworm is dried by using a dryer. A decomposing tank is heated at 121deg.C for 1-2 hours to make a completely sterilized condition. The inside of the tank is cooled up to 30deg.C and pH is fixed as 7. Sterilized enzyme is decomposed in the tank at 50-55deg.C for 72 hours for decomposing 50% of the silkworm. The activity of the enzyme is eliminated by heating the tank at 100deg.C for 1-2 hours. The temperature of the tank is cooled to 30deg.C after eliminating the activity of the enzyme. Remaining silkworm is filtered and collected for reusing in a next decomposing step. The filtrate is stirred for 30 minutes by using active carbons and the active carbons are eliminated by using a filter presser and a micro filter. The filtered solution is concentrated under the temperature of below 50deg.C. The concentrate is dried to get light yellowish powder of silk amino acid and peptide. The powder is packed in a stick or a bottle.

摘要翻译： 目的：提供通过使用蚕丝制造丝氨基酸和丝肽的酶水解方法，明显减少受污染物质的排放，防止工作场所的事故。 构成：去除蛹后，将高质量的蚕丝切成3份，用纯水洗净。 将水和NaHCO 3 5％溶液以10:1的比例混合并在100℃下煮沸2小时进行一次洗涤。 将NaHCO 3 1％溶液在100℃下混合并煮沸1小时进行二次洗涤以除去丝氨酸和残留的NaHCO 3。 通过使用烘干机干燥切丝的蚕。 将分解罐在121℃加热1-2小时以使其完全灭菌。 罐内冷却至30℃，pH值固定为7.灭菌后的酶在50-55℃下分解72小时，分解50％的蚕。 通过在100℃下加热罐1-2小时来消除酶的活性。 消除酶的活性后，将罐的温度冷却至30℃。 剩余的蚕被过滤并收集用于在下一个分解步骤中重新使用。 通过使用活性炭将滤液搅拌30分钟，通过使用过滤器压力机和微滤器除去活性炭。 将过滤的溶液在低于50℃的温度下浓缩。 将浓缩物干燥，得到丝氨酸和肽的淡黄色粉末。 粉末包装在棍子或瓶子里。

公开(公告)号：KR1020000036770A

公开(公告)日：2000-07-05

申请号：KR1020000015945

申请日：2000-03-28

申请人： 주식회사 아미노젠

发明人： 주육현

IPC分类号： A61K36/15 ,  A61P39/06

摘要： PURPOSE: A novel bio-material named antoflavan obtained from the skin of Pinus maritima removes noxious element in a human body and helps resistance against damage caused by blood vessel, skin injury, inflammation or free radical and also removes pain and inflammation by stabilizing collagen of skin cell layer. CONSTITUTION: A preparing method of antoflavin composition comprises the following steps of: harvesting 20-30 year old Pinus maritima; obtaining the skin of Pinus maritima by using roll cutting then drying with hot wind at 50-60°C in Dry-oven to reduce the water content to less than 7 %; grinding the dried material into powder by using ball mixer; adding ethanol of 96 % in ground material and extracting; distilling the extracted solvent by using vacuum condenser; absorbing the condensed solution by using vacuum then isolating; filtering with sodium chloride; extracting the solution five times by using ethylacetate then vacuum condensed; absorbing the condensed solution into ethanol then filtering; vacuum drying at the temperature of minus 20°C. The material prepared is puckery, bright yellowish brown powder and can be melt in ethanol and acetone and can not be melt in benzene, chloroform and ether, and absorbs 256 plus or minus 280 nm of UV spectrum.

摘要翻译： 目的：从马尾松皮肤获得的名为“黄酮”的新型生物材料消除人体中的有害元素，有助于抵抗由血管，皮肤损伤，炎症或自由基引起的损伤，并通过稳定胶原蛋白来消除疼痛和炎症 皮肤细胞层。 构成：一种黄曲霉素组成的制备方法，包括以下步骤：收获20-30岁的马尾松; 通过使用辊切割获得马​​尾松皮肤，然后在干燥炉中用50-60℃的热风干燥以将水分降至小于7％; 使用球磨机将干燥的物料粉碎成粉末; 将96％的乙醇加入研磨物中并萃取; 用真空冷凝器蒸馏除去溶剂; 通过真空吸收冷凝溶液，然后分离; 用氯化钠过滤; 用乙酸乙酯萃取5次，然后真空浓缩; 将浓缩的溶液吸收到乙醇中然后过滤; 在-20℃的温度下进行真空干燥。 制备的材料是白葡萄酒，明亮的黄棕色粉末，可以在乙醇和丙酮中熔融，不能在苯，氯仿和乙醚中熔融，并吸收256加或减去280纳米的紫外光谱。

公开(公告)号：KR1020000007053A

公开(公告)日：2000-02-07

申请号：KR1019990052008

申请日：1999-11-22

申请人： 주식회사 아미노젠

发明人： 주육현

IPC分类号： A23L1/29

摘要： PURPOSE: A manufacturing method for a functional food is provided to be substituted for a meal, to realize a dietary treatment, to be used for promoting a metabolism, and to supply a nutrition. CONSTITUTION: A manufacturing method contains: a step for washing a raw material containing a silk amino acid, peptide element by using a purified water for eliminating an impurity and for selecting the raw material with an excellent quality; hot air drying the selected agricultural and marine raw product for eliminating moisture; eliminating the moisture by using a rapid freeze drying method at -40°C for minimizing destruction of vitamin; powdering the freeze dried raw material into 150-180 mesh by using a crusher; and mixing the powdered raw material for being suitable to a standard for agitating by using an agitator. Therefore, the silk fresh provision including the silk amino acid, peptide element can be used for the substitution for the meal and can be used for preventing a fatness caused by overeating.

摘要翻译： 目的：提供功能性食品的制造方法代替膳食，实现饮食治疗，用于促进新陈代谢，提供营养。 构成：一种制造方法，其特征在于，包括：通过使用净化水清洗含有丝氨基酸的原料的肽元素的步骤，除去杂质，选择原料优良的步骤; 热风干燥选用农副产品消除水分; 通过在-40℃下使用快速冷冻干燥方法消除水分，以最大限度地减少维生素的破坏; 使用破碎机将冷冻干燥原料粉碎成150-180目; 并且通过使用搅拌器将粉末状原料混合以适合用于搅拌的标准。 因此，丝绸氨基酸，肽元素的丝绸新鲜配方可用于替代膳食，可用于防止由暴饮暴食引起的肥胖。

公开(公告)号：KR1020000006718A

公开(公告)日：2000-02-07

申请号：KR1019990046730

申请日：1999-10-26

申请人： 주식회사 아미노젠

发明人： 주육현

IPC分类号： A23L1/227

摘要： PURPOSE: A manufacturing method for a silk amino acid and peptide salt as a functional seasoning is provided to acidifying hydrolyze a fibroin and other proteins being existed in a cocoon. CONSTITUTION: A manufacturing method is composed of: hydrolyzing a selected and cut cocoon in a temperature of 100-110°C for 24-48 hours by using 1.5-2 N of hydrochloric acid solution for obtaining an acidic hydrolyzing solution of a silk protein material; attaching a coloring material to the neutral silk protein acidic hydrolyzing solution by using an active carbon; eliminating a deposited material of acidic amino acid and the active carbon by using a micro filter for obtaining the colorless-light yellow color of transparent silk protein acidic hydrolyzing solution; and concentrating the solution by using a vacuum. The manufacturing method can improve the operating process by simplifying and reducing the manufacturing process and save the manufacturing cost.

摘要翻译： 目的：提供丝氨酸和肽盐作为功能性调味料的制造方法，以酸化水解丝素蛋白和存在于茧中的其它蛋白质。 构成：制造方法包括：使用1.5-2N盐酸溶液在100-110℃的温度下水解选择和切割的茧24-48小时，以获得丝蛋白质材料的酸性水解溶液 ; 通过使用活性炭将着色材料附着到中性丝蛋白酸性水解溶液; 通过使用微过滤器去除酸性氨基酸和活性炭的沉积物，得到透明丝蛋白酸性水解溶液的无色浅黄色; 并通过使用真空浓缩溶液。 制造方法可以通过简化和减少制造过程并节省制造成本来改善操作过程。