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    Method and composition for leucyl-tRNA synthetases and derivatives thereof that activate and aminoacylate non-leucine amino acid to tRNA adaptor molecules
    1.
    发明授权
    Method and composition for leucyl-tRNA synthetases and derivatives thereof that activate and aminoacylate non-leucine amino acid to tRNA adaptor molecules 有权
    亮氨酰tRNA合成酶及其衍生物的方法和组合物，其将非亮氨酸氨基酸活化并氨酰化至tRNA衔接子分子

    公开(公告)号：US07785827B2

    公开(公告)日：2010-08-31

    申请号：US10251648

    申请日：2002-09-20

    申请人： Susan A. Martinis ,  James M. Briggs ,  Richard S. Mursinna ,  Keun Woo Lee ,  Tommie L. Lincecum ,  Amy M. Williams ,  Yuxin Zhai

    发明人： Susan A. Martinis ,  James M. Briggs ,  Richard S. Mursinna ,  Keun Woo Lee ,  Tommie L. Lincecum ,  Amy M. Williams ,  Yuxin Zhai

    IPC分类号： C07K14/47 ,  C07H21/04 ,  C12P21/06

    CPC分类号： C12N9/93

    摘要： A method and composition for tRNA synthetases that activate and aminoacylate nonstandard and noncognate amino acids to tRNA adaptor molecules is described that can be used to generate custom designed protein products for uses in medicinal, therapeutic, diagnostic, biotechnology, engineering, and spectroscopy applications. Some tRNA synthetases naturally misactivate and misaminoacylate noncognate amino acids. Many of these tRNA synthetases, including but not limited to leucyl-, isoleucyl-, and valyl-tRNA synthetases, have evolved proofreading and editing mechanisms to correct these mistakes. Inactivation of the enzyme's editing activity allows and facilitates production and accumulation of tRNAs that are misaminoacylated with nonstandard and noncognate amino acids. These misaminoacylated tRNAs can be used to introduce novel amino acids into proteins.

    摘要翻译： 描述了用于将非标准和非认可氨基酸激活和氨基化至tRNA衔接子分子的tRNA合成酶的方法和组合物，其可用于产生用于药物，治疗，诊断，生物技术，工程和光谱学应用的定制设计的蛋白质产物。 一些tRNA合成酶天然失活和非氨基酸化非识别氨基酸。 许多这些tRNA合成酶，包括但不限于亮氨酰，异亮氨酰和缬氨酰-tRNA合成酶，已经发展了校正和编辑机制，以纠正这些错误。 酶的编辑活性的失活允许并促进用非标准和非认知氨基酸错氨基酰化的tRNA的产生和积累。 这些错氨基酰化的tRNA可用于将新的氨基酸引入蛋白质。

    Portable drilling mud system
    4.
    发明授权
    Portable drilling mud system 失效
    便携式钻井泥浆系统

    公开(公告)号：US4474254A

    公开(公告)日：1984-10-02

    申请号：US439395

    申请日：1982-11-05

    申请人： Russell W. Etter ,  James M. Briggs

    发明人： Russell W. Etter ,  James M. Briggs

    IPC分类号： E21B21/06

    CPC分类号： E21B21/06 ,  E21B21/062

    摘要： A portable well drilling mud storage and recirculation unit includes a mud storage tank mounted on an over-the-road semi-trailer having an engine driven circulating pump mounted onboard and adapted to withdraw mud from the tank for circulation to the well and for recirculation through a set of mud agitating nozzles disposed in the bottom of the tank. A mud degassing vessel, a solids separator unit and an additive blending unit are all mounted above the tank. The degassing vessel is supported by hydraulic cylinder actuators for movement between a retracted transport position and a vertically elevated working position.

    摘要翻译： 便携式钻井泥浆储存和再循环单元包括安装在越野半挂车上的泥浆储存罐，其具有安装在船上的发动机驱动的循环泵，并且适于从罐中取出泥浆以循环到井中并用于再循环通过 设置在罐底部的一组泥浆搅拌喷嘴。 泥浆脱气容器，固体分离器单元和添加剂混合单元都安装在罐的上方。 脱气容器由液压缸致动器支撑，用于在缩回运输位置和垂直升高的工作位置之间移动。