公开(公告)号：US07741541B2

公开(公告)日：2010-06-22

申请号：US10563571

申请日：2003-07-07

Applicant: Naveen Chandra Bisht ,  Arun Jagannath ,  Vibha Gupta ,  Pradeep Kumar Burma ,  Deepak Pental

Inventor： Naveen Chandra Bisht ,  Arun Jagannath ,  Vibha Gupta ,  Pradeep Kumar Burma ,  Deepak Pental

IPC: C12N15/82 ,  C12N15/84 ,  C12N15/31 ,  C12N15/55 ,  A01H5/00 ,  A01H5/10

CPC classification number: C12N15/8289

Abstract: A method for obtaining improved fertility restorer lines for male sterile crop plants and a DNA construct for use in said method are disclosed. The invention relates to the simultaneous use of two different gene sequences encoding the same protein product, one being the naturally occurring wild type sequence and the other sequence being generated by modification of the wild type sequence for expression in crop plants by using codon degeneracy to avoid homology between the two sequences at the DNA and mRNA levels, each of the said sequences being placed under independent transcriptional control of different overlapping plant tissue-specific regulatory elements in the same DNA construct.

Abstract translation: 公开了一种用于获得雄性不育作物植物改良的育性恢复系的方法和用于所述方法的DNA构建体。 本发明涉及同时使用编码相同蛋白质产物的两种不同的基因序列，一种是天然存在的野生型序列，另一种是通过使用密码子简并性修饰用于在作物植物中表达的野生型序列产生的另一序列来避免 在DNA和mRNA水平上两个序列之间的同源性，每个所述序列被置于相同DNA构建体中不同重叠的植物组织特异性调节元件的独立转录控制下。

公开(公告)号：US06833494B1

公开(公告)日：2004-12-21

申请号：US09633274

申请日：2000-08-04

Applicant: Deepak Pental ,  Arun Jagannath ,  Panchali Bandyopadhyay ,  Neelakantan Arumugam ,  Vibha Gupta ,  Pradeep Kumar Burma

Inventor： Deepak Pental ,  Arun Jagannath ,  Panchali Bandyopadhyay ,  Neelakantan Arumugam ,  Vibha Gupta ,  Pradeep Kumar Burma

IPC: A10H500

CPC classification number: C12N15/8263 ,  C12N15/8216 ,  C12N15/8289

Abstract: The present invention relates to an Insulator construct for controlling leaky expression of a lethal gene from enhancing functions of a strong constitutive promoter present in the said Insulator construct following integration into the genome of a plant and a method for development of male sterile lines in crop plants using the said Insulator construct.

Abstract translation: 本发明涉及一种绝缘体构建体，用于在整合到植物的基因组中之后，增强存在于所述绝缘体构建体中的强组成型启动子的功能，以及在作物中发育雄性不育系的方法来控制致死基因的渗漏表达 使用所述绝缘体构造。