Method for obtaining improved fertility restorer lines for transgenic male sterile crop plants and a DNA construct for use in said method
    1.
    发明授权
    Method for obtaining improved fertility restorer lines for transgenic male sterile crop plants and a DNA construct for use in said method 有权
    用于获得改良的转基因雄性不育作物的育性恢复系的方法和用于所述方法的DNA构建体

    公开(公告)号:US07741541B2

    公开(公告)日:2010-06-22

    申请号:US10563571

    申请日:2003-07-07

    CPC classification number: C12N15/8289

    Abstract: A method for obtaining improved fertility restorer lines for male sterile crop plants and a DNA construct for use in said method are disclosed. The invention relates to the simultaneous use of two different gene sequences encoding the same protein product, one being the naturally occurring wild type sequence and the other sequence being generated by modification of the wild type sequence for expression in crop plants by using codon degeneracy to avoid homology between the two sequences at the DNA and mRNA levels, each of the said sequences being placed under independent transcriptional control of different overlapping plant tissue-specific regulatory elements in the same DNA construct.

    Abstract translation: 公开了一种用于获得雄性不育作物植物改良的育性恢复系的方法和用于所述方法的DNA构建体。 本发明涉及同时使用编码相同蛋白质产物的两种不同的基因序列,一种是天然存在的野生型序列,另一种是通过使用密码子简并性修饰用于在作物植物中表达的野生型序列产生的另一序列来避免 在DNA和mRNA水平上两个序列之间的同源性,每个所述序列被置于相同DNA构建体中不同重叠的植物组织特异性调节元件的独立转录控制下。

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