公开(公告)号：US07273699B2

公开(公告)日：2007-09-25

申请号：US10206561

申请日：2002-07-25

申请人： Robert H. Lipsky ,  Chiara Mazzanti ,  David Goldman

发明人： Robert H. Lipsky ,  Chiara Mazzanti ,  David Goldman

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6827 ,  C12Q2527/107 ,  C12Q2527/113

摘要： Sequence variations, such as polymorphisms, are detected by detecting differences between or among melting profiles of plural nucleic acids. Melting profiles are produced by observing the nucleic acids during a temperature change over a period of time. If the nucleic acids are diluted into solutions for analysis, the nucleic acid concentrations between or among the solutions can be substantially the same. The melting profiles and sequence variation can be used to identify changes in a wild-type nucleic acid sequence, such as a single-nucleotide polymorphism (SNP), a small insertion or deletion, or a small inversion.

摘要翻译： 通过检测多个核酸的融合谱之间或之间的差异来检测序列变异，例如多态性。 通过在一段时间内在温度变化期间观察核酸来产生熔融曲线。 如果将核酸稀释成用于分析的溶液，则溶液之间或之间的核酸浓度可以基本上相同。 融合谱和序列变异可用于鉴定野生型核酸序列的变化，如单核苷酸多态性（SNP），小插入或缺失或小反转。