公开(公告)号：US06686195B1

公开(公告)日：2004-02-03

申请号：US09937992

申请日：2001-12-13

申请人： Bruno Colin ,  Philippe Cleuziat ,  Patrick Broyer ,  Claude Mabilat ,  Sandra Incardona

发明人： Bruno Colin ,  Philippe Cleuziat ,  Patrick Broyer ,  Claude Mabilat ,  Sandra Incardona

IPC分类号： C12M133

CPC分类号： B01J19/10 ,  B06B3/00 ,  C12M45/02

摘要： An apparatus including at least one sonotrode (2) designed to generate ultrasound of variable power in at least one biological sample (5) containing cells to be lysed, the sample (5) being contained in at least one receptacle (4 or 10) such that the sonotrode (2) is in direct contact with the receptacle(s) (4). Also disclosed is a method for using ultrasound to lyse a biological sample (5) contained in a receptacle (4), which includes placing the receptacle (4) in direct contact with the sonotrode (2), and activating the sonotrode (2) for long enough to lyse the cells in the sample (5) but preserve the DNA and/or RNA molecules released for subsequent operations, e.g. amplification. The invention is particularly applicable in the discipline of molecular biology.

摘要翻译： 一种装置，包括至少一个超声波发生器（2），其被设计成在至少一个含有待裂解细胞的生物样品（5）中产生可变功率的超声波，所述样品（5）包含在至少一个容器（4或10）中， 超声波发生器（2）与容器（4）直接接触。 还公开了一种使用超声波来溶解包含在容器（4）中的生物样品（5）的方法，其包括将容器（4）放置在与超声焊极（2）直接接触的位置，并且激活超声焊极（2）以用于 足以裂解样品（5）中的细胞，但保留释放用于后续操作的DNA和/或RNA分子，例如 放大。 本发明特别适用于分子生物学学科。

公开(公告)号：US07723095B2

公开(公告)日：2010-05-25

申请号：US10343109

申请日：2001-07-26

申请人： Philippe Cleuziat ,  Sandra Incardona ,  Corinne Jay

发明人： Philippe Cleuziat ,  Sandra Incardona ,  Corinne Jay

IPC分类号： C12N1/00

CPC分类号： C12N1/06 ,  C12M47/06

摘要： A method for lysing prokaryotic or eukaryotic cells, or for simultaneous lysis of both, which includes at least three of the following: a mass of active, small-diameter beads corresponding to 50% or less than a mass of active, larger-diameter beads, and/or a total mass of lysing beads (consisting of a single type of bead or a mixture of smaller and larger beads) corresponding to between 50 and 100% of the total mass of the processed biological sample, and/or a lysis time of from 10 to 20 minutes, and/or seven or less non-lysing glass beads to drive the movement of the lysing beads, and/or from five to fifteen non-lysing iron beads to drive the movement of the lysing beads, depending on whether sonication, mechanical vortex centrifugation or magnetic vortex centrifugation is used.

摘要翻译： 用于裂解原核或真核细胞或同时裂解两者的方法，其包括以下至少三种：大量活性的小直径珠子，其对应于活性较大直径珠粒的质量的50％或更小 ，和/或相当于处理的生物样品总质量的50-100％的裂解珠（由单一类型的珠粒或较小和较大珠粒的混合物组成）的总质量，和/或裂解时间 10分钟至20分钟，和/或7个或更少的非裂解玻璃珠，以驱动裂解珠的移动，和/或5至15个非裂解铁珠以驱动溶胞珠的运动，这取决于 是否使用超声处理，机械涡流离心或磁涡旋离心。