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公开(公告)号：US20240279591A1
公开(公告)日：2024-08-22
申请号：US18569642
申请日：2022-06-12
Inventor： Osnat BOHANA-KASHTAN , Oded PINKAS , Irina GOTLIV , Haim TSUBERY , Tal Yoetz KOPELMAN , Neta CHAIM , Noa AVNI , Hanna LIANDRES , Roy GOVRIN , Yonatan MALCA , Marcelle MACHLUF , Orit AMSALEM-BERGELSON
CPC classification number: C12M47/06 , A61K9/48 , C12N5/0068 , C12N2521/00
Abstract: A method of producing spherical particles is provided herein. Also provided are compositions generated thereby and uses thereof in disease diagnosis and treatment.
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公开(公告)号：US12024702B2
公开(公告)日：2024-07-02
申请号：US16336441
申请日：2017-10-09
Applicant: BIOMÉRIEUX
Inventor： Fabien Dachaud , Jacques Dachaud , Frédéric Foucault , Edgar Minassian , David Mosticone , Jean-Claude Raymond
CPC classification number: C12M47/04 , C12M23/14 , C12M23/26 , C12M47/10 , C12Q1/24 , G01N1/40 , G01N1/405 , C12M47/06 , G01N1/4005
Abstract: A device and method preparing a sample that may contain a target microorganism, includes: placing the sample in a container including: a device for the capture and/or concentration of a target microorganism or of a protein therefrom, including a capture and/or concentration support; a receptacle including; inlet and fluid guides, a device controlling the sample volume transferred from the fluid guide to the capture and/or concentration device through the inlet orifice, an outlet orifice; the capture/concentration device being attached to a wall of the receptacle; generating a displacement of all or part of the sample to the fluid guide and a defined sample volume from the fluid guide to the capture and/or concentration device, capturing and/or concentrating, on the capture and/or concentration support, the target microorganism or a protein from the microorganism, the volume of sample crossing the capture and/or concentration support then exiting again through the outlet orifice.
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公开(公告)号：US11903970B2
公开(公告)日：2024-02-20
申请号：US17132634
申请日：2020-12-23
Applicant: Regenexx, LLC
Inventor： Christopher J. Centeno , Neven Steinmetz , Ian Stemper , Dustin Berger
CPC classification number: A61K35/19 , A61K45/06 , C12M47/06 , C12N5/0018 , C12N5/0644 , C12M35/04 , C12N2523/00 , G01N33/86
Abstract: Apparatus, kit and method embodiments are disclosed herein that provide for the production of a modified autologous platelet mixture at a patient bedside for contemporaneous reinjection to the patient using a platelet lysis apparatus. Representative platelet lysis apparatus may include a housing supporting a sample tube and a thermal mass element connected to or near the sample tube. The thermal mass element may be separable from the housing, and/or the sample tube may be separable from the thermal mass element.
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公开(公告)号：US20230323483A1
公开(公告)日：2023-10-12
申请号：US18203584
申请日：2023-05-30
Applicant: SHAHEEN INNOVATIONS HOLDING LIMITED
Inventor： Imad Lahoud , Mohammed Alshaiba Saleh Ghannam Almazrouei , Sajid Bhatti , Jeff Machovec , Clement Lamoureux
CPC classification number: C12Q1/70 , B01L7/52 , C12M47/06 , B01L2300/1883 , B01L2300/1805
Abstract: A disease screening device (100) comprising a substrate (101) and a sonication chamber (102) formed on the substrate (101). The sonication chamber (102) is provided with an ultrasonic transducer (105) which generates ultrasonic waves to lyse cells in a sample fluid within the sonication chamber (102). The device (100) comprises a reagent chamber (111) formed on the substrate (101) for receiving a liquid PCR reagent. The device (100) comprises a controller (23) which controls the ultrasonic transducer (105) and a heating arrangement (128) which is provided on the substrate (101). The device (100) further comprises a detection apparatus which detects the presence of an infectious disease, such as COVID-19 disease.
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公开(公告)号：US11719608B2
公开(公告)日：2023-08-08
申请号：US17581940
申请日：2022-01-23
Applicant: S2 Genomics, Inc.
Inventor： Stevan Bogdan Jovanovich , Frank Zaugg , Kaiwan Chear , Roger McIntosh , Nathan Pereira
CPC classification number: G01N1/31 , C12M47/04 , C12M47/06 , C12N1/06 , C12N1/066 , C12N15/1003 , C12Q1/6851 , G01N1/286 , G01N2001/2866 , C12Q1/6851 , C12Q2525/161 , C12Q2525/179 , C12Q2563/179 , C12Q2565/514
Abstract: This disclosure provides methods for producing a sample of subcellular organelles, particularly nuclei, from a tissue. In some embodiments, this disclosure provides a method of processing a tissue sample involves performing enzymatic/chemical disruption of tissue in a chamber to produce disrupted tissue comprising released cells and/or nuclei and debris; separating the released cells and/or nuclei from the debris therein; and moving the released cells and/or nuclei. In some instances, the method comprises mechanical disruption of the tissue sample.
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Patent Agency Ranking
公开(公告)号：US11667979B2
公开(公告)日：2023-06-06
申请号：US17693209
申请日：2022-03-11
Applicant: SHAHEEN INNOVATIONS HOLDING LIMITED
Inventor： Imad Lahoud , Mohammed Alshaiba Saleh Ghannam Almazrouei , Sajid Bhatti , Jeff Machovec , Clement Lamoureux
CPC classification number: C12Q1/70 , B01L7/52 , C12M47/06 , B01L2300/1805 , B01L2300/1883
Abstract: A disease screening device (100) comprising a substrate (101) and a sonication chamber (102) formed on the substrate (101). The sonication chamber (102) is provided with an ultrasonic transducer (105) which generates ultrasonic waves to lyse cells in a sample fluid within the sonication chamber (102). The device (100) comprises a reagent chamber (111) formed on the substrate (101) for receiving a liquid PCR reagent. The device (100) comprises a controller (23) which controls the ultrasonic transducer (105) and a heating arrangement (128) which is provided on the substrate (101). The device (100) further comprises a detection apparatus which detects the presence of an infectious disease, such as COVID-19 disease.
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公开(公告)号：US20190212233A1
公开(公告)日：2019-07-11
申请号：US16301249
申请日：2017-11-29
Applicant: Stevan JOVANOVICH , Kaiwan CHEAR , Nathan PEREIRA , Frank ZAUGG , Roger MCINTOSH , S2 Genomics, Inc.
Inventor： Stevan JOVANOVICH , Frank ZAUGG , Kaiwan CHEAR , Roger MCINTOSH , Nathan PEREIRA
IPC: G01N1/31 , C12M1/00 , G01N1/28 , C12Q1/6851
CPC classification number: G01N1/31 , C12M47/04 , C12M47/06 , C12N1/06 , C12N1/066 , C12N15/1003 , C12Q1/6851 , G01N1/286 , G01N2001/2866 , C12Q2525/161 , C12Q2525/179 , C12Q2563/179 , C12Q2565/514
Abstract: A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells or nuclei in suspension or subcellular components including nucleic acids. In some embodiments, the titer of dissociated cells is monitored at intervals and the viability determined. In some embodiments, the processing is adjusted according to the measurements of the titer and viability. In some embodiments, the single-cells or nuclei in suspension are washed and resuspended in the buffer or media of choice. In some embodiments, the conditions are chosen to produce nuclei. In other embodiments, the single-cells or nuclei are purified by affinity paramagnetic bead processing. In some embodiments, matched bulk nucleic acid to the single-cells is produced. In other embodiments, single-cell libraries, or nuclei libraries, or matched bulk libraries, or bulk libraries are produced. The single cells or nuclei can then be further processed by FACS, DNA sequencing, mass spectrometry, fluorescence, or other methods. In other embodiments, the tissue processing is integrated with an analytical system to produce a sample-to-answer system such as a tissue-to-genomics system.
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公开(公告)号：US20190001344A1
公开(公告)日：2019-01-03
申请号：US15965128
申请日：2018-04-27
Applicant: The General Hospital Corporation , GPB Scientific, LLC
Inventor： Lotien Richard Huang , Thomas A. Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
CPC classification number: B03C1/30 , B01L3/502746 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0472 , B01L2400/0487 , B01L2400/086 , B03C1/32 , B03C2201/18 , B33Y80/00 , C12M47/04 , C12M47/06 , G01N1/40 , G01N1/4077 , G01N33/5044 , G01N2035/00237 , Y10T137/0318 , Y10T137/8593 , Y10T436/25 , Y10T436/25375
Abstract: The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.
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公开(公告)号：US10072258B2
公开(公告)日：2018-09-11
申请号：US14910530
申请日：2014-07-30
Applicant: Robert Bosch GmbH
Inventor： Bernd Faltin , Franz Laermer , Jochen Rupp , Peter Rothacher , Christian Dorrer , Sebastian Berning
CPC classification number: C12N15/1003 , C07H1/06 , C12M47/04 , C12M47/06 , C12N1/06 , C12N1/066 , C12N1/08 , C12N15/1017 , G01N29/00
Abstract: A method for preparing a sample of biological materials containing target cells and accompanying cells for extracting nucleic acids of the target cells includes accumulating the target cells of the sample by separating the target cells or the accompanying cells from the sample. The method also includes digesting the target cells via chemical and/or physical lysis in order to produce a target cell lysate containing the nucleic acids of the target cells. The method furthermore includes purifying the nucleic acids from the target cell lysate in order to extract the nucleic acids of the target cells.
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公开(公告)号：US20170268978A1
公开(公告)日：2017-09-21
申请号：US15505343
申请日：2015-09-11
Applicant: ANTHROGENESIS CORPORATION
Inventor： Lauren M. DEPALMA , Daniel DEWITT , Li REN
IPC: G01N15/06
CPC classification number: G01N15/0618 , C12M41/36 , C12M47/06 , G01N2015/0069 , G01N2015/0693
Abstract: Provided are methods for quantifying and/or detecting sub-visible particulates in cell cultures. Specifically, the methods comprise a step of breaking down, e.g., lysing, cells in a cell culture. The methods can further comprising filtering the cell culture through a filter. Further provided are methods of quantifying sub-visible particulates that do not pass through the filter using a microscope.
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