公开(公告)号：US4560665A

公开(公告)日：1985-12-24

申请号：US613893

申请日：1984-05-24

申请人： Taiji Nakae ,  Eisaku Ryo

发明人： Taiji Nakae ,  Eisaku Ryo

IPC分类号： G01N33/48 ,  A61K9/127 ,  G01N21/64 ,  G01N33/52 ,  G01N21/77

CPC分类号： A61K9/1277 ,  G01N21/6428 ,  Y10S436/829

摘要： This method of measuring membrane fusions between liposomes made of phospholipid is characterized by the combination of ionophores, fluorescent dyes sensitive to the membrane potential (interior negative) and porins which are the pore-forming membrane proteins. By this method, the extent of liposome fusion can be determined easily and accurately.

摘要翻译： 这种测量由磷脂制成的脂质体之间的膜融合的方法的特征在于离子载体，对膜电位敏感的荧光染料（内部阴性）和作为成孔膜蛋白的孔蛋白的组合。 通过该方法，可以容易且准确地测定脂质体融合的程度。

公开(公告)号：US20110171334A1

公开(公告)日：2011-07-14

申请号：US12782928

申请日：2010-05-19

申请人： Naomi YAMASHITA ,  Takeru IIZUKA ,  Taiji NAKAE ,  Sachiko SATAKE

发明人： Naomi YAMASHITA ,  Takeru IIZUKA ,  Taiji NAKAE ,  Sachiko SATAKE

IPC分类号： A01N63/02 ,  A01P1/00

CPC分类号： C12P1/04 ,  C07G11/00

摘要： An object is to provide a more potent novel antimicrobial active substance, which is derived from a naturally occurring substance and has a broad antimicrobial spectrum, and an antimicrobial active substance DM0507 obtained by a production process comprising the following steps (a) to (d): (a) culturing Bacillus subtilis; (b) collecting the supernatant from the obtained culture; (c) collecting the precipitate formed by adjusting the pH of the supernatant to 3 or lower; and (d) performing extraction from the precipitate with ethanol is provided.

摘要翻译： 目的是提供一种更有效的新型抗微生物活性物质，其衍生自天然存在的物质并具有广泛的抗菌谱，以及通过包括以下步骤（a）至（d）的生产方法获得的抗微生物活性物质DM0507， ：（a）培养枯草芽孢杆菌; （b）从所得培养物中收集上清液; （c）通过将上清液的pH调节至3以下而收集形成的沉淀物; 并提供（d）用乙醇从沉淀物中萃取。

公开(公告)号：US20080193577A1

公开(公告)日：2008-08-14

申请号：US11911690

申请日：2005-11-04

申请人： Naomi Yamashita ,  Takeru Iizuka ,  Taiji Nakae ,  Sachiko Satake

发明人： Naomi Yamashita ,  Takeru Iizuka ,  Taiji Nakae ,  Sachiko Satake

IPC分类号： A61K35/00 ,  A23L3/34 ,  C12N1/20 ,  A61P43/00

CPC分类号： C12P1/04 ,  C07G11/00

摘要： An object is to provide a more potent novel antimicrobial active substance, which is derived from a naturally occurring substance and has a broad antimicrobial spectrum, and an antimicrobial active substance DM0507 obtained by a production process comprising the following steps (a) to (d):(a) culturing Bacillus subtilis; (b) collecting the supernatant from the obtained culture;(c) collecting the precipitate formed by adjusting the pH of the supernatant to 3 or lower; and(d) performing extraction from the precipitate with ethanol is provided.

摘要翻译： 目的是提供一种更有效的新型抗微生物活性物质，其衍生自天然存在的物质并具有广泛的抗菌谱，以及通过包括以下步骤（a）至（d）的生产方法获得的抗微生物活性物质DM0507， ：（a）培养枯草芽孢杆菌; （b）从所得培养物中收集上清液; （c）通过将上清液的pH调节至3以下而收集形成的沉淀物; 并提供（d）用乙醇从沉淀物中萃取。