公开(公告)号：US07341869B2

公开(公告)日：2008-03-11

申请号：US10328813

申请日：2002-12-23

申请人： William R. A. Osborne ,  Nagarajan Ramesh

发明人： William R. A. Osborne ,  Nagarajan Ramesh

IPC分类号： C12N5/06 ,  C12N15/00 ,  A61K38/28

CPC分类号： C12N5/069 ,  A61K35/12 ,  A61K38/28 ,  A61K48/00 ,  C12N2510/02 ,  Y10S424/81 ,  Y10S514/866

摘要： The present invention provides an isolated population of cells containing an expressible nucleic acid encoding proinsulin containing a proinsulin cleavage site and a glucose-regulated expressible nucleic acid encoding a protease capable of cleaving the proinsulin cleavage site to produce insulin. The invention also provides an isolated population of cells which further express a hexosamine synthetic pathway enzyme. The invention additionally provides vectors containing an expressible nucleic acid encoding proinsulin containing a proinsulin cleavage site and a glucose-regulated expressible nucleic acid encoding a protease capable of cleaving the proinsulin cleavage site to produce insulin. The invention further provides a method of treating or preventing diabetes by implanting into an individual cells coexpressing proinsulin containing a proinsulin cleavage site and a glucose-regulated protease capable of cleaving the proinsulin cleavage site to produce insulin.

摘要翻译： 本发明提供了分离的细胞群，其含有含有胰岛素原切割位点的可表达核酸的胰岛素原，以及编码能够切割胰岛素原切割位点产生胰岛素的蛋白酶的葡萄糖调节的可表达核酸。 本发明还提供进一步表达己糖胺合成途径酶的分离的细胞群。 本发明另外提供含有编码含有胰岛素原切割位点的胰岛素原的可表达核酸的载体和编码能够切割胰岛素原切割位点产生胰岛素的蛋白酶的葡萄糖调节的可表达核酸。 本发明还提供了一种通过植入含有胰岛素原切割位点的共表达胰岛素原的单个细胞和能够切割胰岛素原切割位点产生胰岛素的葡萄糖调节的蛋白酶来治疗或预防糖尿病的方法。

公开(公告)号：US06759037B1

公开(公告)日：2004-07-06

申请号：US09323738

申请日：1999-06-01

申请人： William R. A. Osborne ,  Nagarajan Ramesh

发明人： William R. A. Osborne ,  Nagarajan Ramesh

IPC分类号： A01N6300

CPC分类号： C12N5/069 ,  A61K35/12 ,  A61K38/28 ,  A61K48/00 ,  C12N2510/02 ,  Y10S424/81 ,  Y10S514/866

摘要： The present invention provides an isolated population of cells containing an expressible nucleic acid encoding proinsulin containing a proinsulin cleavage site and a glucose-regulated expressible nucleic acid encoding a protease capable of cleaving the proinsulin cleavage site to produce insulin. The invention also provides an isolated population of cells which further express a hexosamine synthetic pathway enzyme. The invention additionally provides vectors containing an expressible nucleic acid encoding proinsulin containing a proinsulin cleavage site and a glucose-regulated expressible nucleic acid encoding a protease capable of cleaving the proinsulin cleavage site to produce insulin. The invention further provides a method of treating or preventing diabetes by implanting into an individual cells coexpressing proinsulin containing a proinsulin cleavage site and a glucose-regulated protease capable of cleaving the proinsulin cleavage site to produce insulin.

摘要翻译： 本发明提供了分离的细胞群，其含有含有胰岛素原切割位点的可表达核酸的胰岛素原，以及编码能够切割胰岛素原切割位点产生胰岛素的蛋白酶的葡萄糖调节的可表达核酸。 本发明还提供进一步表达己糖胺合成途径酶的分离的细胞群。 本发明另外提供含有编码含有胰岛素原切割位点的胰岛素原的可表达核酸的载体和编码能够切割胰岛素原切割位点产生胰岛素的蛋白酶的葡萄糖调节的可表达核酸。 本发明还提供了一种通过植入含有胰岛素原切割位点的共表达胰岛素原的单个细胞和能够切割胰岛素原切割位点产生胰岛素的葡萄糖调节的蛋白酶来治疗或预防糖尿病的方法。

公开(公告)号：US06537806B1

公开(公告)日：2003-03-25

申请号：US09185852

申请日：1998-11-04

申请人： William R. A. Osborne ,  Nagarajan Ramesh

发明人： William R. A. Osborne ,  Nagarajan Ramesh

IPC分类号： C12N500

CPC分类号： C12N5/069 ,  A61K35/12 ,  A61K38/28 ,  A61K48/00 ,  C12N2510/02 ,  Y10S424/81 ,  Y10S514/866

摘要： The present invention provides an isolated population of cells containing an expressible nucleic acid encoding proinsulin containing a proinsulin cleavage site and a glucose-regulated expressible nucleic acid encoding a protease capable of cleaving the proinsulin cleavage site to produce insulin. The invention also provides an isolated population of cells which further express a hexosamine synthetic pathway enzyme. The invention additionally provides vectors containing an expressible nucleic acid encoding proinsulin containing a proinsulin cleavage site and a glucose-regulated expressible nucleic acid encoding a protease capable of cleaving the proinsulin cleavage site to produce insulin. The invention further provides a method of treating or preventing diabetes by implanting into an individual cells coexpressing proinsulin containing a proinsulin cleavage site and a glucose-regulated protease capable of cleaving the proinsulin cleavage site to produce insulin.

摘要翻译： 本发明提供了分离的细胞群，其含有含有胰岛素原切割位点的可表达核酸的胰岛素原，以及编码能够切割胰岛素原切割位点产生胰岛素的蛋白酶的葡萄糖调节的可表达核酸。 本发明还提供进一步表达己糖胺合成途径酶的分离的细胞群。 本发明另外提供含有编码含有胰岛素原切割位点的胰岛素原的可表达核酸的载体和编码能够切割胰岛素原切割位点产生胰岛素的蛋白酶的葡萄糖调节的可表达核酸。 本发明还提供了一种通过植入含有胰岛素原切割位点的共表达胰岛素原的单个细胞和能够切割胰岛素原切割位点产生胰岛素的葡萄糖调节的蛋白酶来治疗或预防糖尿病的方法。