公开(公告)号：US08263347B2

公开(公告)日：2012-09-11

申请号：US12738684

申请日：2008-10-20

Applicant: Hirohito Tsubouchi ,  Hirofumi Uto ,  Takeshi Okanoue ,  Yo-ichi Ishida ,  Yuko Sato ,  Masayuki Sudo

Inventor： Hirohito Tsubouchi ,  Hirofumi Uto ,  Takeshi Okanoue ,  Yo-ichi Ishida ,  Yuko Sato ,  Masayuki Sudo

IPC: G01N33/53

CPC classification number: G01N33/6893 ,  G01N2333/8139 ,  G01N2800/085

Abstract: Disclosed are: a marker for the diagnosis of a liver disease, which can determine the disease in a simple manner; an antibody directed against the marker; a diagnostic agent; a diagnosis method; and a method for marker detection in blood or serum. Proteome analysis revealed that quantities of the full-length kininogen and three partial peptides thereof (sequence A: position-440 to position-456, sequence B: position-439 to position-456, and sequence C: position-438 to position-456) in sera of patients with non-alcoholic fatty liver disease are significantly different from those in sera of healthy individuals; and a diagnostic agent and a detecting method for the non-alcoholic fatty liver disease that can be conveniently used for medical examination are established. The use of a combination of a kininogen-based marker and a C4-based marker (the full length sequence or partial peptides thereof) enables identification of chronic hepatitis and an asymptomatic virus carrier, as well as non-alcoholic fatty liver disease.

Abstract translation: 公开了一种用于诊断肝脏疾病的标志物，其可以以简单的方式确定疾病; 针对标记的抗体; 诊断剂; 诊断方法; 以及血液或血清中标记物检测的方法。 蛋白质组分析显示，全长激肽原及其三个部分肽（序列A：位置440至位置-456，序列B：位置-439至位置-456，序列C：位置-438至位置-456） ）与非酒精性脂肪性肝病患者的血清显着不同于健康人血清; 并建立了可以方便地用于体格检查的非酒精性脂肪肝疾病的诊断剂和检测方法。 使用基于激肽原的标记物和基于C4的标记物（其全长序列或其部分肽）的组合能够鉴定慢性肝炎和无症状病毒载体以及非酒精性脂肪肝疾病。

公开(公告)号：US20110129859A1

公开(公告)日：2011-06-02

申请号：US12738684

申请日：2008-10-20

Applicant: Hirohito Tsubouchi ,  Hirofumi Uto ,  Takeshi Okanoue ,  Yo-ichi Ishida ,  Yuko Sato ,  Masayuki Sudo

Inventor： Hirohito Tsubouchi ,  Hirofumi Uto ,  Takeshi Okanoue ,  Yo-ichi Ishida ,  Yuko Sato ,  Masayuki Sudo

IPC: G01N33/53 ,  C07K7/08 ,  C07K14/435 ,  C07K16/18

CPC classification number: G01N33/6893 ,  G01N2333/8139 ,  G01N2800/085

Abstract: Disclosed are: a marker for the diagnosis of a liver disease, which can determine the disease in a simple manner; an antibody directed against the marker; a diagnostic agent; a diagnosis method; and a method for marker detection in blood or serum. Proteome analysis revealed that quantities of the full-length kininogen and three partial peptides thereof (sequence A: position-440 to position-456, sequence B: position-439 to position-456, and sequence C: position-438 to position-456) in sera of patients with non-alcoholic fatty liver disease are significantly different from those in sera of healthy individuals; and a diagnostic agent and a detecting method for the non-alcoholic fatty liver disease that can be conveniently used for medical examination are established. The use of a combination of a kininogen-based marker and a C4-based marker (the full length sequence or partial peptides thereof) enables identification of chronic hepatitis and an asymptomatic virus carrier, as well as non-alcoholic fatty liver disease.

Abstract translation: 公开了：用于诊断肝脏疾病的标志物，其可以以简单的方式确定疾病; 针对标记的抗体; 诊断剂; 诊断方法; 以及血液或血清中标记物检测的方法。 蛋白质组分析显示，全长激肽原及其三个部分肽（序列A：位置440至位置-456，序列B：位置-439至位置-456，序列C：位置-438至位置-456） ）与非酒精性脂肪性肝病患者的血清显着不同于健康人血清; 建立了可以方便地用于体格检查的非酒精性脂肪肝疾病的诊断剂和检测方法。 使用基于激肽原的标记物和基于C4的标记物（其全长序列或其部分肽）的组合能够鉴定慢性肝炎和无症状病毒载体以及非酒精性脂肪肝疾病。