VIRUS INDUCED GENE SILENCING (VIGS) FOR FUNCTIONAL ANALYSIS OF GENES IN COTTON
    1.
    发明申请
    VIRUS INDUCED GENE SILENCING (VIGS) FOR FUNCTIONAL ANALYSIS OF GENES IN COTTON 有权
    用于基因功能分析的病毒基因沉默(VIGS)

    公开(公告)号:US20120096595A1

    公开(公告)日:2012-04-19

    申请号:US13376902

    申请日:2010-06-10

    摘要: The invention relates to the functional analysis of genes in cotton by employing the Virus Induced Gene Silencing (VIGS) method. More specifically this method induces gene silencing in cotton with the help of the Tobacco Rattle Virus (TRV) vectors RNA1 and RNA2 and phenotypic effects on the cotton plant can be analysed Moreover this invention also provides transient expression vector TRV RNA2 in order to transiently express genes in cotton plants and plant tissue under the influence of a strong subgenomic promoter.

    摘要翻译: 本发明涉及使用病毒诱导基因沉默(VIGS)方法对棉花中基因的功能分析。 更具体地说,这种方法在烟草叮咬病毒(TRV)载体RNA1和RNA2的帮助下诱导棉花中的基因沉默,并且可以分析对棉花植物的表型效应。此外,本发明还提供瞬时表达载体TRV RNA2以瞬时表达基因 在棉花植物和植物组织中受到强亚基因子启动子的影响。

    Virus induced gene silencing (VIGS) for functional analysis of genes in cotton
    2.
    发明授权
    Virus induced gene silencing (VIGS) for functional analysis of genes in cotton 有权
    病毒诱导的基因沉默(VIGS)用于棉花基因的功能分析

    公开(公告)号:US08853493B2

    公开(公告)日:2014-10-07

    申请号:US13376902

    申请日:2010-06-10

    IPC分类号: A01H1/00 C12N15/82

    摘要: The invention relates to the functional analysis of genes in cotton by employing the Virus Induced Gene Silencing (VIGS) method. More specifically this method induces gene silencing in cotton with the help of the Tobacco Rattle Virus (TRV) vectors RNA1 and RNA2 and phenotypic effects on the cotton plant can be analysed Moreover this invention also provides transient expression vector TRV RNA2 in order to transiently express genes in cotton plants and plant tissue under the influence of a strong subgenomic promoter.

    摘要翻译: 本发明涉及使用病毒诱导基因沉默(VIGS)方法对棉花中基因的功能分析。 更具体地说,这种方法在烟草叮咬病毒(TRV)载体RNA1和RNA2的帮助下诱导棉花中的基因沉默,并且可以分析对棉花植物的表型效应。此外,本发明还提供瞬时表达载体TRV RNA2以瞬时表达基因 在棉花植物和植物组织中受到强亚基因子启动子的影响。