公开(公告)号：US20160145593A1

公开(公告)日：2016-05-26

申请号：US14903194

申请日：2014-07-17

Applicant: ACADEMIA SINICA

Inventor： Meng-Chiao HO ,  Shih-Hsiung WU ,  Wan-Ling WU

IPC: C12N9/52 ,  C12P21/06 ,  C07K14/39 ,  C12N15/52

CPC classification number: C12N9/52 ,  C07K14/39 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/50 ,  C12N15/52 ,  C12P21/06 ,  C12Y304/21

Abstract: A fusion gene encoding M. taiwanensis WR-220 keratinase is disclosed. The fusion comprises: (a) a first DNA sequence encoding a protein secretion signal peptide, located at the N-terminus of the fusion gene; (b) a second DNA sequence encoding an inhibitory domain of M. taiwanensis WR-220 keratinase, linked in translation frame with the first DNA sequence; and (c) a third DNA sequence encoding a catalytic domain of M. taiwanensis WR-220 keratinase, linked in translation frame with the second DNA sequence, wherein the fusion gene is a non-naturally occurring chimeric DNA. Also disclosed are a method for preparation of the catalytic domain of M. taiwanensis WR-220 keratinase, and use of the M. taiwanensis WR-220 keratinase.

Abstract translation: 公开了一种编码台湾华ensis WR-220角蛋白酶的融合基因。 融合物包含：（a）编码位于融合基因N末端的蛋白质分泌信号肽的第一DNA序列; （b）编码与第一DNA序列连接的翻译框架的台湾华ensis WR-220角蛋白酶的抑制结构域的第二DNA序列; 和（c）编码与第二DNA序列连接的翻译框架的台湾华南WR-220角蛋白酶的催化结构域的第三DNA序列，其中融合基因是非天然存在的嵌合DNA。 还公开了一种制备M. taiwanensis WR-220角蛋白酶的催化结构域的方法，并使用了台湾华氏WR-220角蛋白酶。