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公开(公告)号：US12084667B2
公开(公告)日：2024-09-10
申请号：US17584028
申请日：2022-01-25
Applicant: Impossible Foods Inc.
Inventor： Smita Shankar , Martin Andrew Hoyt
IPC: C12N15/81 , C07K14/39 , C07K14/415 , C07K14/805 , C12N1/16 , C12N15/63
CPC classification number: C12N15/815 , C07K14/39 , C07K14/415 , C07K14/805 , C12N1/16 , C12N15/635 , C12N15/81
Abstract: Methods and materials for genetically engineering methylotrophic yeast are provided.
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公开(公告)号：US20240115727A1
公开(公告)日：2024-04-11
申请号：US16669191
申请日：2019-10-30
Applicant: University of Virginia Patent Foundation
Inventor： Matthew J. Lazzara , Benjamin Purow , Evan K. Day
CPC classification number: A61K47/6901 , A61K47/6415 , A61P35/00 , C07K14/035 , C07K14/39 , C12N15/86 , C12Y207/01021 , C12Y305/04001
Abstract: Disclosed are compositions and methods for treating a disease or disorder such as cancer in a subject in need thereof. In some aspects, the method comprises administering to the subject a vector comprising a first nucleic acid sequence encoding a promoter operably linked to each of a second nucleic acid sequence encoding a therapeutic polypeptide, and a third nucleic acid sequence encoding a peptide domain that is stabilized when phosphorylated by kinase activity in a target tissue. The kinase activity can be elevated extracellular regulated kinase (ERK) activity.
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公开(公告)号：US20240018464A1
公开(公告)日：2024-01-18
申请号：US18335717
申请日：2023-06-15
Applicant: CARGILL, INCORPORATED
Inventor： Kenneth R. FINLEY , Holly JESSEN , Erin Kathleen MARASCO , Thomas William MCCMULLIN , Ana NEGRETE-RAYMOND , Amit VAS
Abstract: Less-than-diploid I. orientalis cells are produced. The cells have at least one unpaired chromosome and may be haploid, i.e., are missing one member of each pair of chromosomes that are present in the wild-type strains. The less-than-diploid cells are useful fermentation strains, performing similarly to diploid strains that are otherwise similarly engineered. The less-than-diploid strains can be mated to produce diploids, which themselves are useful fermentation strains. The less-than-diploid strains are also useful as host strains for producing further genetically modified strains that can be less-than-diploid or mated to produce diploids.
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公开(公告)号：US11702679B2
公开(公告)日：2023-07-18
申请号：US17388338
申请日：2021-07-29
Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHA
Inventor： Junji Ito , Toru Onishi , Nobuki Tada , Rie Hirao
Abstract: This invention is intended to improve the ethanol fermentation ability of a yeast strain having xylose-metabolizing ability with the use of a mutant gene encoding a mutant protein comprising a consensus sequence comprising a substitution of amino acid in the 30th position in SEQ ID NO: 1, amino acid in the 43rd position in SEQ ID NO: 4, and amino acid in the 31st position in SEQ ID NO: 7 with other amino acid residues.
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5.发明公开公开(公告)号：US20230192781A1
公开(公告)日：2023-06-22
申请号：US16766845
申请日：2017-12-13
Inventor： Min GUO , Lingxuan JIANG , Zhuxia ZHENG , Xue YU
CPC classification number: C07K14/39 , C12N15/67 , C12N15/815
Abstract: What provided is a method for regulating in vitro biosynthesis activity by knocking-out of a nuclease system, comprising: screening five nucleases among numerous nucleases, and performing down-regulation or knocking-out on one of the five nucleases (e.g., EXN53). The method can improve the stability of nucleic acid and the protein production efficiency of an in vitro protein synthesis system.
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Patent Agency Ranking
公开(公告)号：US20170349906A1
公开(公告)日：2017-12-07
申请号：US15678342
申请日：2017-08-16
Applicant: Impossible Foods Inc.
Inventor： Smita Shankar , Martin Andrew Hoyt
IPC: C12N15/81 , C07K14/415
CPC classification number: C12N15/815 , C07K14/39 , C07K14/415 , C07K14/805 , C12N1/16 , C12N15/635 , C12N15/81
Abstract: Methods and materials for genetically engineering methylotrophic yeast are provided.
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公开(公告)号：US20170327850A1
公开(公告)日：2017-11-16
申请号：US15523218
申请日：2015-10-29
Inventor： Hal ALPER , John BLAZECK , Andrew HILL
CPC classification number: C12P7/44 , C07K14/39 , C07K14/47 , C12N1/16 , C12N9/1025 , C12N9/1205 , C12N9/78 , C12N9/88 , C12N15/815 , C12Y203/03001 , C12Y305/04006 , C12Y401/01006
Abstract: Genetically engineered oleaginous fungi (e.g., engineered Yarrowia lipolytica) are provided for use in itaconic acid production. In some aspects, the engineered fungi comprise a transgene for expression of a cis-aconitic acid decarboxylase (CAD) enzyme and, optionally, one or more further genetic modifications. Methods and culture systems for production of itaconic acid using such fungi are also provided.
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公开(公告)号：US20170218032A1
公开(公告)日：2017-08-03
申请号：US15367135
申请日：2016-12-01
Applicant: Adnan HALIM , Hiren J. JOSHI , Ida Signe Bohse LARSEN , Yanqui YUAN , Henrik CLAUSEN
Inventor： Adnan HALIM , Hiren J. JOSHI , Ida Signe Bohse LARSEN , Yanqui YUAN , Henrik CLAUSEN
IPC: C07K14/395 , C07K14/39 , C12P21/02
CPC classification number: C07K14/395 , C07K7/08 , C07K14/00 , C07K14/39 , C12N9/1051 , C12P21/00 , C12P21/02
Abstract: The present disclosure provides, inter alia, compositions and methods for modulating and/or utilizing nucleocytoplasmic alpha-mannosyltransferase enzyme activity in yeast, e.g., to modulate the performance of the yeast in fermentation, respiration, bioproduction and/or bioprocessing. The disclosure also provides, inter alia, modified eukaryotic organisms comprising modulated nucleocytoplasmic alpha-mannosyltransferase enzyme activity, and uses of such modified eukaryotic organisms.
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公开(公告)号：US20170137742A1
公开(公告)日：2017-05-18
申请号：US15319686
申请日：2015-06-16
Applicant: NESTE OYJ
Inventor： Arto HEISKA , Annika MALM , Jukka-Pekka PASANEN
CPC classification number: C11B1/08 , A23K20/147 , C07K14/39 , C10L1/02 , C10L5/445 , C10L9/086 , C10L2290/08 , C10L2290/26 , C10L2290/544 , C10L2290/547 , C11B1/02 , C11B1/10 , C11B1/108 , C11B1/14 , C12P7/6463 , Y02E50/10 , Y02E50/30
Abstract: A method is disclosed for recovering lipids from microbial biomass, and for producing lipids. In the method, an aqueous suspension includes fermentation broth containing oleaginous yeast biomass which is subjected to hydrothermal treatment and lipids are recovered from the biomass by solvent extraction.
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公开(公告)号：US20170037418A1
公开(公告)日：2017-02-09
申请号：US15335132
申请日：2016-10-26
Applicant: LONZA LTD.
Inventor： DIETHARD MATTANOVICH , BRIGITTE GASSER , MICHAEL MAURER , ROLAND PRIELHOFER , JOACHIM KLEIN , JANA WENGER
CPC classification number: C12N15/815 , C07K14/39 , C07K16/00 , C07K2317/55 , C12N9/0008 , C12N15/635 , C12N15/67 , C12N15/81 , C12P21/00 , C12Y102/01012
Abstract: A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate or at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.
Abstract translation: 一种通过培养包含表达构建体的重组真核生物细胞系（包括可调节启动子和编码所述启动子的转录控制下的POI的核酸分子）的重组真核生物细胞系的方法，包括以下步骤：a）培养细胞 与抑制启动子的基础碳源连接，b）用有限量的补充碳源培养细胞系，抑制启动子以与转录因子相比以转录速率或至少15％产生POI pGAP启动子，和c）生产和回收POI; 并进一步分离可调节的启动子和相应的表达系统。
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