公开(公告)号：US20150111237A1

公开(公告)日：2015-04-23

申请号：US14399051

申请日：2013-05-07

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Carrie A. Graham ,  Allen E. Eckhardt

IPC: C12Q1/34

CPC classification number: C12Q1/34 ,  G01N33/542 ,  G01N33/582

Abstract: The invention relates to methods for enzymatic detection of biotinidase activity. In certain aspects, the invention provides methods for bench-based enzymatic detection of biotinidase activity. In other aspects, the invention provides methods for bench-based enzymatic detection in droplets in oil. The droplet-based methods may be performed in an automated manner using digital microfluidics technology on a droplet actuator device. The enzymatic assays for biotinidase activity may be used for newborn testing for biotinidase deficiency, and may be combined with other droplet-based enzymatic assays in a panel of tests for newborn testing.

Abstract translation: 本发明涉及酶检测生物素酶活性的方法。 在某些方面，本发明提供了用于基于酶的酶检测生物素酶活性的方法。 在其它方面，本发明提供了用于油中液滴的基于酶的酶检测方法。 基于液滴的方法可以使用液滴致动器装置上的数字微流体技术以自动方式进行。 用于生物素酶活性的酶测定可用于生物素酶缺陷的新生儿测试，并且可以在新生儿测试的一组测试中与其他基于液滴的酶测定组合。

公开(公告)号：US10731199B2

公开(公告)日：2020-08-04

申请号：US14359177

申请日：2012-11-20

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Carrie A. Graham ,  Allen E. Eckhardt

IPC: C12Q1/32

Abstract: Aspects of embodiments may include methods for automated enzymatic detection of glucose-6-phosphate dehydrogenase (G6PD) activity. Aspects of embodiments may include methods for enzymatic detection of G6PD activity in droplets in oil. Aspects of embodiments may include a system including a droplet actuator. Aspects of embodiments may include a treatment method.

公开(公告)号：US20150267242A1

公开(公告)日：2015-09-24

申请号：US14439255

申请日：2013-10-30

Applicant: ADVANCED LIQUID LOGIC, INC

Inventor： Nora J. Foegeding ,  Carrie A. Graham ,  Allen E. Eckhardt

IPC: C12Q1/32

CPC classification number: C12Q1/32 ,  G01N33/6893 ,  G01N2333/90206 ,  G01N2800/04 ,  G01N2800/7085

Abstract: A method for conducting medium-chain acyl-CoA dehydrogenase (MCAD) and very-long-chain acyl-CoA dehydrogenase (VCAD) enzymatic activity assays is provided. The method may include, but is not limited to, preparing a sample; preparing an enzyme-specific substrate/reagent mixture; mixing an aliquot of the prepared sample with an aliquot of the enzyme-specific substrate/reagent mixture; reading absorbance in the range of about 600 nm; incubating the prepared sample and enzyme-specific substrate/reagent mixture; and reading absorbance in the range of about 600 nm at various time intervals.

Abstract translation: 提供了一种用于进行中链酰基辅酶A脱氢酶（MCAD）和非常长链的酰基辅酶A脱氢酶（VCAD）酶活性测定的方法。 该方法可以包括但不限于制备样品; 制备酶特异性底物/试剂混合物; 将制备的样品的等分试样与酶特异性底物/试剂混合物的等分试样混合; 读取吸光度在约600nm范围内; 孵育制备的样品和酶特异性底物/试剂混合物; 并以各种时间间隔读取约600nm范围内的吸光度。

公开(公告)号：US20140335069A1

公开(公告)日：2014-11-13

申请号：US14359177

申请日：2012-11-20

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Carrie A. Graham ,  Allen E. Eckhardt

IPC: C12Q1/32

CPC classification number: C12Q1/32 ,  G01N2333/904

Abstract: Aspects of embodiments may include methods for automated enzymatic detection of glucose-6-phosphate dehydrogenase (G6PD) activity. Aspects of embodiments may include methods for enzymatic detection of G6PD activity in droplets in oil. Aspects of embodiments may include a system including a droplet actuator. Aspects of embodiments may include a treatment method.

Abstract translation: 实施方案的各方面可以包括葡萄糖-6-磷酸脱氢酶（G6PD）活性的自动酶检测方法。 实施方案的各方面可以包括用于在油中的液滴中酶检测G6PD活性的方法。 实施例的方面可以包括包括液滴致动器的系统。 实施方案的各方面可以包括治疗方法。