公开(公告)号：US20220243222A1

公开(公告)日：2022-08-04

申请号：US17609303

申请日：2020-05-04

Applicant: AMGEN INC.

Inventor： Pavan Ghattyvenkatakrishna ,  Hedieh Barkhordarian ,  Kristine Marie Daris ,  Charilyn Tejamo

IPC: C12N15/85 ,  C12N9/00 ,  C12N15/62 ,  C07K16/06

Abstract: Inventions disclosed herein relates to vectors and expression systems for producing heteromeric recombinant proteins such as monoclonal antibodies. Vectors and expression systems disclosed herein are based on the finding that the selectable marker glutamine synthetase can be divided into two fragments at selected amino acid positions of the glutamine synthetase polypeptide, and the two fragments can internet and/or associate to form a monomer and then a functional multimeric glutamine synthetase protein. Z